Human PD-1 ELISA Kit PicoKine®

Product Name

Human PD-1 ELISA Kit PicoKine®

View all PD-1 ELISA kits

SKU/Catalog Number

EK0959

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PD-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human PDCD1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human PD-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0959)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: L25-Q167

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0959 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0959 is reactive to PDCD1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0959 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of PD-1

Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded by the PDCD1 gene. This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. By fluorescence in situ hybridization, the PDCD1 gene is mapped to 2q37.3. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.

Kit Components

Catalog Number	Description	Quantity
EK0959-CAP	Anti-Human PDCD1 Pre-coated 96-well strip microplate	1
EK0959-ST	Human PDCD1 Standard	2 vials, 10 ng/tube
EK0959-DA	Human PDCD1 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.002	0.054	0.102	0.189	0.360	0.660	1.042	1.907

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect PD-1, Dilution ratio of 1:1, concentration in serum and plasma is 80-120 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PD-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of PD-1 in ELISA kits from four different production batches/lots.

Gene/Protein Information For PDCD1 (Source: Uniprot.Org, NCBI)

Alternative Names

Programmed cell death protein 1;Protein PD-1;hPD-1;CD279;PDCD1;PD1; PDCD1 CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1 programmed cell death 1 programmed cell death protein 1|programmed cell death 1 protein|protein PD-1|systemic lupus erythematosus susceptibility 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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