Anti-Human CD40 Monoclonal Antibody, Flow Validated

Product Name

Anti-Human CD40 Monoclonal Antibody, Flow Validated

View all CD40/TNFRSF5 Antibodies

SKU/Catalog Number

FC00068

Size

100ug, 500ug

Form

Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA

Description

Boster Bio Anti-Human CD40 Monoclonal Antibody Unconjugated Conjugated, Flow Validated (Catalog# FC00068). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Human CD40 Monoclonal Antibody, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00068)

Host

Mouse

Contents

PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: G28-5

Isotype

IgG1,k

Immunogen

Human tonsillar lymphocytes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC00068 is reactive to CD40 in Cynomolgus monkey, Human, Baboon, Rhesus monkey

Reconstitution

Calculated molecular weight

30619 MW

Background of CD40/TNFRSF5

CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

FC00068 is guaranteed for Flow Cytometry Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For CD40 (Source: Uniprot.org, NCBI)

Alternative Names

Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CDw40;CD40;CD40;TNFRSF5; CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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