CD40 Antibodies

Tumor necrosis factor receptor superfamily member 5 (CD40)

Receptor for TNFSF5/CD40LG.

Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, resulting in induction of immunoglobulin secretion.

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Gene Information

Human
Gene Name:	CD40
Uniprot:	P25942
Entrez:	958

Family

Belongs to:
No superfamily

Alternative Names

B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

Molecular Weight

Mass (kDA):
30.619 kDA

Genomic Context

Human
Location:	20q13.12
Sequence:	20; NC_000020.11 (46118242..46129858)

Tissue Specificity

B-cells and in primary carcinomas.

Subcellular Localizations

[Isoform I]: Cell membrane; Single-pass type I membrane protein.; [Isoform II]: Secreted.

Diseases

• Neoplasms
• Inflammation
• Malignant Neoplasms
• Autoimmune Reaction
• Tissue Adhesions
• Lymphoma
• Autoimmune Diseases
• Leukemia
• Immunologic Deficiency Syndromes
• B-cell Lymphomas
• Carcinoma
• Autoimmunity
• Malignant Paraganglionic Neoplasm

• Infective Disorder
• Decreased Immunologic Activity [pe]
• Atherosclerosis
• Arthritis
• Lymphoid Leukemia
• Chronic Lymphocytic Leukemia
• Rheumatoid Arthritis

Interacting Proteins

• Otud7b
• TRAF2
• TRAF3
• TRAF6

Pathways

• Secretion
• Immune Response
• Cell Activation
• Cell Proliferation
• Pathogenesis
• Cytokine Production
• Cell Death
• Cell Differentiation
• T Cell Proliferation
• Cell Cycle
• T Cell Activation
• B Cell Activation
• Cell Growth

• B Cell Proliferation
• Isotype Switching
• Cell Maturation
• Inflammatory Response
• Cytokine Secretion
• Sensitization
• Cell Adhesion

PTMs

• Phosphorylation
• Cleavage
• Methylation
• Acetylation
• Glycosylation
• Dephosphorylation
• Phosphorothioation
• Biotinylation
• Ubiquitination
• Reduction

• Oxidation
• Nitration
• Demethylation
• Myristoylation
• Acylation
• Ribosylation
• Palmitoylation
• Nitrosylation
• Carboxylation
• Deamination

Research Areas

• Adaptive Immunity
• Apoptosis
• Asthma
• Cancer
• Hematopoietic Stem Cell Markers

• Immunology
• Signal Transduction
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CD40

Best Uses Of The CD40 Marker

The CD40 Marker is a highly specific antigen which is typically associated with allergies. Researchers use it to detect allergies, and also to detect inflammatory reactions and tumor growth. There are many uses for the CD40 marker. Each has its own advantages and disadvantages. In this article, we'll look at the most popular uses and benefits of this antibody. It is useful to all scientists and researchers around the world.

Flow Cytometry

Flow Cytometry is a popular technique used in immunohistochemistry. The CD40 marker, which detects the presence CD40 in human cells is a highly specific antibody. Flow cytometry experiments require specialized sample preparation and special analysis techniques to yield high-quality data. This guide will help you prepare your samples correctly and maximize the effectiveness of your flow-cytometry experiments.

Flow Cytometry has become a popular method to detect cancerous cells. Its rapid detection speed makes it ideal for the screening of tumor cells. It also is useful for studying other diseases, like AIDS. Flow Cytometry utilizes fluorescently conjugated antibodies to identify specific cell structures. Cells that are fluorescently stained react with the beam of a laser to emit a pulse of light for a specific time. PMTs detect this light. The intensity of cells is determined by the size of each pulse. The experiment will have more channels when there is more fluorescence.

The kit contained detection antibody mix, PE-streptavidin, and beads. FlowJo software was used to analyze the results. In FlowJo the beads were classified by size and fluorescence intensities (MFI). The median fluorescence intensity (MFI) of each population was determined. This number is then used to determine the concentrations of cytokine.

Boster Bio CD40 marker can be used to identify highly specific and robust methods. It recognizes CD40 that is expressed on human primary B cells. It is also used in flow cytometry as an adjunct to conventional immunohistochemistry. The Boster Bio CD40 marker is available at an affordable price and is accessible from various online vendors. You can find out more about the Boster Bio CD40 marker below.

The data gathered from Flow Cytometry with the Boster BIO CD40 marker is easily accessible and allows thorough analysis of the CD40 level in human B cells. The Boster BIO CD40 marker can be utilized with all cytometry instruments. It also serves a variety of functions. It is useful for analyzing specific cellular processes and immune-related diseases. It is readily available to all scientists and is highly recommended for research and development.

ELISA

The CD40 marker belongs to the group of proteins known as caspases. These proteins are activated and respond to various apoptosis-related stimuli. Common triggers include DNA damage cell surface stimuli, and mitochondrial dysfunction. Boster Bio has antibodies that recognize this protein. Boster Bio is an excellent source of high-quality antibodies.

The CD40 molecule, a surface-based receptor, is found on mature B cells and monocytes as well as on the epithelium thymicus. It is a member of the tumor necrosis factor receptor superfamily. The ligand it binds to is a member the TNF gene familia. The CD40 ligand has been shown to inhibit B-cell lymphomas, as well as transformed cells of mesenchymal source. Boster Bio has validated all its antibodies using multiple methods.

Cell activation

The CD40 Marker protein is found on activated B cells and is expressed by dendritic cell. The protein is located in dendritic cell dendritic cells and is involved in activation of T cells. The protein is also referred to by the names CD154and T-BAM and TRAP. Its most popular function is to participate in the transcription of the T cell receptor.

Boster Bio Anti CD40L/Cd40lg is part of Picoband(tm). It has been tested for IHC and ELISA as well as WB applications. It binds human, mouse, and rat CD40L and is beneficial for studying cell activation. Its high affinity makes it ideal for WB and ELISA applications.

The CD40 marker is expressed in antigen-presenting cells and DCs. It triggers a range of changes in these cells, including the upregulation of MHC class II and co-stimulatory molecules. Antigen-presenting cells and DCs also respond to CD40 signaling with antibodies. Also, CD40-induced B cells death and GM-CSF production happen.

Numerous studies have revealed that CD40 antagonist antibodies can stimulate antigen-presenting cells (APCs), in murine models. This can boost the immune response in humans. The lack of a suitable drug makes it difficult to translate these findings into clinical practice. In the meantime, phase I studies of an antagonist CD40 monoclonal antibody have shown promising results.

When activated by CD40L CD40L triggers B cells produce more IL-12/IFN-g than DCs that are not activated by CD40L. This cytokine is also able to enhance DC survival. One of the most well-known methods for activating cells is the stimulation of CD40L. Although CD40-stimulated DCs possess more effects than those stimulated by other types of B cells, RANKL stimulation of B cells has significant effects on both types of DCs.

Allergy testing

Allergy testing using the CD40 label has been proven to be able to detect allergic reactions in patients with chronic ocular diseases. Its accuracy can be evaluated by determining the percentage of T cells that are allergen-specific (TCR-T) within the sample. Patients with severe allergic reactions could be at greater risk of developing a condition which could affect their eyes. This test has the added benefit of identifying allergic rhinitis an extremely common cause of chronic eye diseases.

Allergy testing using the CD40-positive T-cells can aid in the diagnosis of seasonal allergic rhinitis. This is a kind of IgE-mediated allergy. The condition is most likely to manifest in the spring and fall when pollen levels are high. Seasonal allergic rhinitis is characterized by itching and reddening of the eyes. The signs and symptoms vary depending on the allergen that caused the reaction and the place and time at which they were experienced.

Although eosinophils primarily are T cells, they play a significant role in local inflammation. As antigen-presenting cellsthey can present processed antigens to T cells and trigger the inflamatory chain of events. They also express HLA-DR, MHC class II molecules and human leukocyte antigen. Eosinophils also have higher levels of HLA-DR, and MHC class II molecules in EoE.

Incredibly, there are a variety of factors that influence the response of T-cells to allergens. The most important thing isn't the amount of allergens, but the time and duration of exposure. Genetic factors should be taken into consideration. The test is not intended to be used to replace allergy testing. It is sensitive, however, it can detect allergens. A study on mice showed that it is less sensitive than a blood test.