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Product Info Summary
| SKU: | EKC1653 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
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Product info
Product Name
TALL-2 Colorimetric Cell-Based ELISA
See all APRIL/TNFSF13 products
SKU/Catalog Number
EKC1653
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The TALL-2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TALL-2 protein expression profile in cells. The kit can be used for measuring the relative amounts of TALL-2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TALL-2.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
TALL-2 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1653)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1653 is reactive to TNFSF13 in Human, Mouse, Rat samples
Application Guarantee
EKC1653 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-TALL-2 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HeLa/COLO205 cells
Protein Target Info & Infographic
Gene/Protein Information For TNFSF13 (Source: Uniprot.Org, NCBI)
Gene Name
TNFSF13
Full Name
Tumor necrosis factor ligand superfamily member 13
Weight
27433 MW
Superfamily
tumor necrosis factor family
Alternative Names
Tumor necrosis factor ligand superfamily member 13;A proliferation-inducing ligand;APRIL;TNF- and APOL-related leukocyte expressed ligand 2;TALL-2;TNF-related death ligand 1;TRDL-1;CD256;TNFSF13;APRIL, TALL2, ZTNF2;UNQ383/PRO715; TNFSF13 APRIL, CD256, TALL-2, TALL2, TNLG7B, TRDL-1, UNQ383/PRO715, ZTNF2 TNF superfamily member 13 tumor necrosis factor ligand superfamily member 13|TNF- and APOL-related leukocyte expressed ligand 2|a proliferation-inducing ligand|tumor necrosis factor (ligand) superfamily, member 13|tumor necrosis factor ligand 7B|tumor necrosis factor superfamily member 13|tumor necrosis factor-like protein ZTNF2|tumor necrosis factor-related death ligand-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFSF13, check out the TNFSF13 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFSF13: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TALL-2 Colorimetric Cell-Based ELISA (EKC1653)
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