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Product Info Summary
| SKU: | PA1019 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD40 Antibody Picoband®
View all CD40/TNFRSF5 Antibodies
SKU/Catalog Number
PA1019
Size
100 μg/vial
Form
lyophilized
Description
Boster Bio Anti-CD40 Antibody catalog # PA1019. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD40 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1019)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CD40.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1019 is reactive to Cd40 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
130 kDa
Calculated molecular weight
32093 MW
Background of CD40/TNFRSF5
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1019 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-CD40 antibody, PA1019, Western blotting
Lane 1: Recombinant Mouse CD 40 Protein 10ng
Lane 2: Recombinant Mouse CD 40 Protein 5ng
Lane 3: Recombinant Mouse CD 40 Protein 2.5ng
Protein Target Info & Infographic
Gene/Protein Information For Cd40 (Source: Uniprot.org, NCBI)
Gene Name
Cd40
Full Name
Tumor necrosis factor receptor superfamily member 5
Weight
32093 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CD40;Cd40;Tnfrsf5; CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd40, check out the Cd40 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD40 Antibody Picoband® (PA1019)
Hello CJ!
PA1019 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng Hx, Li Hn, Geng Js, Ohe R, Yu Xy, E Xq, Ye F, Yang Sr, Kato T, Zhang L, Ishida A, Ohta N, Jin Xm, Kakehata S, Geng Js, Yamakawa M. Transl Res. 2015 Sep;166(3):281-91. Doi: 10.1016/J.Trsl.2015.04.004. Epub 2015 Apr 17. Decreased Expression Of ...
Peng Yd, Cheng Lx, Zeng Qt, Shi Y, Bao Xx, Mao Xb, Ji Qw, Li Sn, Guo M, Liang Zs, Wu Tc. Cell Biol Int. 2010 Mar 24;34(5):447-53. Doi: 10.1042/Cbi20090301. The Role Of Rantes As A Crucial Downstream Cytokine In Calcineurin-Dependent Vsmc Apoptosis...
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-CD40 Antibody Picoband®
Question
We are currently using anti-CD40 antibody PA1019 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
The anti-CD40 antibody (PA1019) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-13
Question
Could I conjugate or label this antibody? Keywords: conjugation, fluorescent dye, antibody labeling, fluorescence labeling
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
It is not recommendeded to label the antibody because it contains BSA. Please contact us at [email protected] for BSA-free antibody products.
Boster Scientific Support
Answered: 2020-01-30
Question
We have observed staining in mouse ovary. Do you have any suggestions? Is anti-CD40 antibody supposed to stain ovary positively?
G. Johnson
Verified customer
Asked: 2013-08-02
Answer
According to literature ovary does express CD40. According to Uniprot.org, CD40 is expressed in left coronary artery, kidney, ovary, leukocyte, lymphoma urinary bladder carcinoma, among other tissues. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161
Boster Scientific Support
Answered: 2013-08-02
Question
My team were well pleased with the WB result of your anti-CD40 antibody. However we have observed positive staining in left coronary artery isoform i: cell membrane using this antibody. Is that expected? Could you tell me where is CD40 supposed to be expressed?
K. Bhatt
Verified customer
Asked: 2013-04-30
Answer
From literature, left coronary artery does express CD40. Generally CD40 expresses in isoform i: cell membrane. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161
Boster Scientific Support
Answered: 2013-04-30
