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Product Info Summary
| SKU: | EK0702 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. |
Product info
Product Name
Human CD40/TNFRSF5 ELISA Kit PicoKine®
View all CD40/TNFRSF5 ELISA kits
SKU/Catalog Number
EK0702
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD40/TNFRSF5 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD40 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD40/TNFRSF5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0702)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E21-R193
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0702 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0702 is reactive to CD40 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
Application Guarantee
EK0702 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD40/TNFRSF5
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0702-CAP | Anti-Human CD40 Pre-coated 96-well strip microplate | 1 |
| EK0702-ST | Human CD40 Standard | 2 vials, 10 ng/tube |
| EK0702-DA | Human CD40 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.010 | 0.035 | 0.077 | 0.141 | 0.221 | 0.419 | 0.997 | 1.720 |
Data Example Images
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Human CD40/TNFRSF5 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD40/TNFRSF5, Dilution ratio of 1:1, concentration in serum and plasma is around 90 pg/ml..
Some articles we found to cite concentrations of CD40/TNFRSF5 in samples: 15004517 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD40/TNFRSF5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 32 | 167 | 443 | 32 | 177 | 404 |
| Standard deviation | 1.66 | 9.18 | 34.99 | 1.92 | 10.79 | 38.78 |
| CV (%) | 5.2% | 5.5% | 7.9% | 6% | 6.1% | 9.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD40/TNFRSF5 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 32 | 30 | 36 | 34 | 33 | 2.23 | 6.7% |
| Sample 2 | 167 | 185 | 189 | 177 | 179 | 8.41 | 4.6% |
| Sample 3 | 443 | 505 | 507 | 519 | 493 | 29.64 | 6% |
Protein Target Info & Infographic
Gene/Protein Information For CD40 (Source: Uniprot.Org, NCBI)
Gene Name
CD40
Full Name
Tumor necrosis factor receptor superfamily member 5
Weight
30619 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CDw40;CD40;CD40;TNFRSF5; CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD40, check out the CD40 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD40: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD40/TNFRSF5 ELISA Kit PicoKine® (EK0702)
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Recommended Resources
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human CD40/TNFRSF5 ELISA Kit PicoKine®
Question
Q: which procedure should I follow in order to thaw whole blood sample for CD40 ELISA after freezing?
A. Phillips
Verified customer
Asked: 2020-01-12
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD40 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-01-12
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CD40 using the EK0702 Human CD40 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-10-07
Question
Is the Human CD40/TNFRSF5 ELISA Kit PicoKine antibody been tried to measure dog CD40?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Our Human CD40/TNFRSF5 ELISA Kit PicoKine has not been tested in canine samples. In terms of homology there is only 70% of human and canine sequences.
Boster Scientific Support
Answered: 2019-06-14
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD40 in Human saliva? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD40 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-06-05
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-05-30
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-01-28
Question
Q: Can CD40 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
W. Robinson
Verified customer
Asked: 2019-01-11
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-01-11
Question
Q: if the enzyme conjugated CD40 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD40 antigen?
Verified Customer
Verified customer
Asked: 2018-04-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-04-29
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2017-01-17
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-01-17
