Product Info Summary
SKU: | EZ0702 |
---|---|
Size: | For 5 plates, 96 wells each |
Reactive Species: | Human |
Application: | ELISA |
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Product info
Product Name
Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
SKU/Catalog Number
EZ0702
Size
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Description
Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CD40 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0702)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E21-R193
Assay Range
15.6 pg/ml - 1,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ0702 is reactive to CD40 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
Application Guarantee
EZ0702 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material |
---|---|---|---|
EZ0702-CA | Rabbit anti- human CD40 polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
EZ0702-DA | Biotinylated goat anti- human CD40 polyclonal antibody (Detection Antibody) | 500 µl, 5 µg/mL (recommended dilution 1:100) | |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
EK0702-ST | Lyophilized recombinant human CD40 standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.01 | 0.077 | 0.147 | 0.221 | 0.419 | 0.997 | 1.62 | 2.394 |
Data Example Images
![ez0702 2 ELISA human cd40 tnfrsf5 ez set elisa kit diy antibody pairs ez0702 2 ELISA human cd40 tnfrsf5 ez set elisa kit diy antibody pairs](https://www.bosterbio.com/media/catalog/product/antibody/ez0702-2-ELISA-human-cd40-tnfrsf5-ez-set-elisa-kit-diy-antibody-pairs.jpg)
Click image to see more details
Human CD40/TNFRSF5 EZ Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 93 | 325 | 538 | 101 | 357 | 529 |
Standard deviation | 4.8 | 19.2 | 28.5 | 6.9 | 26.4 | 32.8 |
CV (%) | 5.2% | 5.9% | 5.3% | 6.8% | 7.4% | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For CD40 (Source: Uniprot.Org, NCBI)
Gene Name
CD40
Full Name
Tumor necrosis factor receptor superfamily member 5
Weight
30619 MW
Alternative Names
B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5 CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface antigen CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD40, check out the CD40 Infographic
![CD40 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD40: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0702)
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Customer Q&As
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1 Customer Q&As for Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
Question
Has anybody tested this EZ0702 antibody to measure dog CD40?
Verified Customer
Verified customer
Asked: 2016-03-23
Answer
Unfortunately, the antibody EZ0702 has not been tested in canine samples and its homology has about 70% only with the human and canine sequences.
Boster Scientific Support
Answered: 2016-03-23