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Product Info Summary
| SKU: | PA1019-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CD40 Antibody Picoband®
View all CD40/TNFRSF5 Antibodies
SKU/Catalog Number
PA1019-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD40 Antibody catalog # PA1019-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD40 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1019-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CD40.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1019-1 is reactive to CD40 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
30619 MW
Background of CD40/TNFRSF5
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1019-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Daudi whole cell, human Raji whole cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD40 using anti-CD40 antibody (PA1019-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40 antigen affinity purified polyclonal antibody (Catalog # PA1019-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40 at approximately 40 kDa. The expected band size for CD40 is at 30 kDa.
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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-CD40 antibody (PA1019-1).
Overlay histogram showing THP-1 cells stained with PA1019-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD40 Antibody (PA1019-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CD40 (Source: Uniprot.org, NCBI)
Gene Name
CD40
Full Name
Tumor necrosis factor receptor superfamily member 5
Weight
30619 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CDw40;CD40;CD40;TNFRSF5; CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD40, check out the CD40 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD40 Antibody Picoband® (PA1019-1)
Hello CJ!
PA1019-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng Hx, Li Hn, Geng Js, Ohe R, Yu Xy, E Xq, Ye F, Yang Sr, Kato T, Zhang L, Ishida A, Ohta N, Jin Xm, Kakehata S, Geng Js, Yamakawa M. Transl Res. 2015 Sep;166(3):281-91. Doi: 10.1016/J.Trsl.2015.04.004. Epub 2015 Apr 17. Decreased Expression Of ...
Peng Yd, Cheng Lx, Zeng Qt, Shi Y, Bao Xx, Mao Xb, Ji Qw, Li Sn, Guo M, Liang Zs, Wu Tc. Cell Biol Int. 2010 Mar 24;34(5):447-53. Doi: 10.1042/Cbi20090301. The Role Of Rantes As A Crucial Downstream Cytokine In Calcineurin-Dependent Vsmc Apoptosis...
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Customer Q&As
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4 Customer Q&As for Anti-CD40 Antibody Picoband®
Question
How come some of your antibodies have more than one catalog numbers for the same target? One example is anti-CD40 antibody which has two catalog numbers: PA1019, PA1019-1.
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
It is because for some targets we have at least two antibodies with different immunogen sequences to target different epitopes and/or species. For the case of anti-CD40 antibody, PA1019 has an immunogen N-terminus of mouse CD40 (22-36 aa, QCVTCSDKQYLHDGQ), while the immunogen for PA1019-1 is N-terminus of human CD40 (20-37 aa, PEPPTACREKQYLINSQC).
Boster Scientific Support
Answered: 2020-02-21
Question
We have been able to see staining in human ovary. What should we do? Is anti-CD40 antibody supposed to stain ovary positively?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
From literature ovary does express CD40. From Uniprot.org, CD40 is expressed in left coronary artery, kidney, ovary, leukocyte, lymphoma urinary bladder carcinoma, among other tissues. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161
Boster Scientific Support
Answered: 2019-08-07
Question
My boss were content with the WB result of your anti-CD40 antibody. However we have been able to see positive staining in left coronary artery isoform i: cell membrane using this antibody. Is that expected? Could you tell me where is CD40 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
From what I have seen in literature, left coronary artery does express CD40. Generally CD40 expresses in isoform i: cell membrane. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161
Boster Scientific Support
Answered: 2018-07-24
Question
We are currently using anti-CD40 antibody PA1019-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
C. Collins
Verified customer
Asked: 2013-12-31
Answer
The anti-CD40 antibody (PA1019-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-12-31
