Product Info Summary
SKU: | CI1039 |
---|---|
Size: | 50μg/25μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ChIP, Dot blot, ELISA, IF, WB |
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Product info
Product Name
Anti-H4K20me1 HIST1H4A Antibody
SKU/Catalog Number
CI1039
Size
50μg/25μl
Form
Liquid
Description
Boster Bio Anti-H4K20me1 HIST1H4A Antibody (Catalog# CI1039). Tested in ChIP, ELISA, Dot blot, WB, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C. For long-term storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Cite This Product
Anti-H4K20me1 HIST1H4A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1039)
Host
Rabbit
Contents
Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Clonality
Polyclonal
Immunogen
This antibody is raised in rabbit against histone H4 containing the monomethylated lysine 20 (H4K20me1), using a KLH-conjugated synthetic peptide.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
CI1039 is reactive to H4C1 in Human
Applications
CI1039 is guaranteed for ChIP, Dot blot, ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
11367 MW
Background of H4C1
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
![ci1039_chip_qpcr_2 anti h4k20me1 antibody ci1039_chip_qpcr_2 anti h4k20me1 antibody](https://www.bosterbio.com/media/catalog/product/antibody/ci1039_chip_qpcr_2-anti-h4k20me1-antibody.jpg)
Click image to see more details
ChIP assays were performed using human HeLa cells, Anti-H4K20me1 polyclonal antibody (Catalog # CI1039) and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the GAPDH promoter and a region located 1 kb upstream of the GAPDH promoter, used as negative controls.
![ci1039_elisa anti h4k20me1 antibody ci1039_elisa anti h4k20me1 antibody](https://www.bosterbio.com/media/catalog/product/antibody/ci1039_elisa-anti-h4k20me1-antibody.jpg)
Click image to see more details
To determine the titer, an ELISA was performed using a serial dilution of Anti-H4K20me1 polyclonal antibody (Catalog # CI1039) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:51,100.
![ci1039_wb anti h4k20me1 antibody ci1039_wb anti h4k20me1 antibody](https://www.bosterbio.com/media/catalog/product/antibody/ci1039_wb-anti-h4k20me1-antibody.png)
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Western blot analysis of H4K20me1 expression in HeLa whole cell lysates (25 μg, lane 1), recombinant histone H2A (1 μg, lane 2), recombinant histone H2B (1 μg, lane 3), recombinant histone H3 (1 μg, lane 4) and recombinant histone H4 (1 μg, lane 5). H4K20me1 was detected using Anti-H4K20me1 polyclonal antibody (Catalog # CI1039) at 1/1000 dilution.
![ci1039_dot_blot ci1039_dot_blot](https://www.bosterbio.com/media/catalog/product/c/i/ci1039_dot_blot.png)
Click image to see more details
A Dot Blot analysis was performed to test the cross reactivity of Anti-H4K20me1 polyclonal antibody (Catalog # CI1039) with peptides containing different modifications of histone H3 and H4 or the unmodified H4K20 sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. This figure shows a high specificity of the antibody for the modification of interest.
Protein Target Info & Infographic
Gene/Protein Information For H4C1 (Source: Uniprot.org, NCBI)
Gene Name
H4C1
Full Name
Histone H4
Weight
11367 MW
Superfamily
histone H4 family
Alternative Names
Histone H4 H4C1 H4-161, H4C12, H4C13, H4C14, H4C15, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4FA, HIST1H4A, H4C1 H4 clustered histone 1 histone H4|H4 histone family, member A|histone 1, H4a|histone cluster 1 H4 family member a|histone cluster 1, H4a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H4C1, check out the H4C1 Infographic
![H4C1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H4C1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-H4K20me1 HIST1H4A Antibody (CI1039)
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