Click image to see more details
Product Info Summary
| SKU: | A00121-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FGF2 Antibody Picoband®
View all FGF basic/FGF2/bFGF Antibodies
SKU/Catalog Number
A00121-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGF2 Antibody Picoband® catalog # A00121-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00121-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FGF2 recombinant protein (Position: P143-S288).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00121-1 is reactive to FGF2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17-21 kDa
Calculated molecular weight
30.77kDa
Background of FGF basic/FGF2/bFGF
FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00121-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry, 2-5 μg/ml
Immunocytochemistry/Immunofluorescence, 5 μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: human SKOV3 whole cell, human Hela whole cell
IHC: human mammary cancer tissue
ICC/IF: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SKOV3 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # A00121-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.
Click image to see more details
Figure 2. IHC analysis of FGF2 using anti-FGF2 antibody (A00121-1).
FGF2 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF2 Antibody (A00121-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IF analysis of FGF2 using anti-FGF2 antibody (A00121-1).
FGF2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FGF2 Antibody (A00121-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1133) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For FGF2 (Source: Uniprot.org, NCBI)
Gene Name
FGF2
Full Name
Fibroblast growth factor 2
Weight
30.77kDa
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB FGF2 BFGF, FGF-2, FGFB, HBGF-2 fibroblast growth factor 2 fibroblast growth factor 2|basic fibroblast growth factor bFGF|fibroblast growth factor 2 (basic)|heparin-binding growth factor 2|prostatropin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF2, check out the FGF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGF2 Antibody Picoband® (A00121-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FGF2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-FGF2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-FGF2 Antibody Picoband®
Question
I was wanting to use your anti-FGF2 antibody for ELISA for mouse hepatoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse hepatoma identification?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
It shows on the product datasheet, A00121-1 anti-FGF2 antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse hepatoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-29
Question
Our team were well pleased with the WB result of your anti-FGF2 antibody. However we have seen positive staining in cartilage tissue secreted. nucleus. note=exported from cells using this antibody. Is that expected? Could you tell me where is FGF2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
Based on literature, cartilage tissue does express FGF2. Generally FGF2 expresses in secreted. nucleus. note=exported from cells. Regarding which tissues have FGF2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 2435575
Hepatoma, Pubmed ID: 1721615, 2538817
Hepatoma, and Placenta, Pubmed ID: 3579930
Boster Scientific Support
Answered: 2019-09-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hepatoma using anti-FGF2 antibody A00121-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-26
Question
We are currently using anti-FGF2 antibody A00121-1 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
The anti-FGF2 antibody (A00121-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-26
Question
My lab would like to test anti-FGF2 antibody A00121-1 on mouse hepatoma for research purposes, then I may be interested in using anti-FGF2 antibody A00121-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Jha
Verified customer
Asked: 2019-04-12
Answer
The products we sell, including anti-FGF2 antibody A00121-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-12
Question
We have observed staining in mouse hepatoma. What should we do? Is anti-FGF2 antibody supposed to stain hepatoma positively?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
From literature hepatoma does express FGF2. From Uniprot.org, FGF2 is expressed in cartilage tissue, hepatoma, blood, hepatoma placenta, among other tissues. Regarding which tissues have FGF2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 2435575
Hepatoma, Pubmed ID: 1721615, 2538817
Hepatoma, and Placenta, Pubmed ID: 3579930
Boster Scientific Support
Answered: 2018-06-22
Question
Is this A00121-1 anti-FGF2 antibody reactive to the isotypes of FGF2?
V. Krishna
Verified customer
Asked: 2018-03-20
Answer
The immunogen of A00121-1 anti-FGF2 antibody is E. coli-derived human FGF2 recombinant protein (Position: P143-S288). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-20
Question
Would anti-FGF2 antibody A00121-1 work for ELISA with hepatoma?
Verified Customer
Verified customer
Asked: 2018-02-27
Answer
According to the expression profile of hepatoma, FGF2 is highly expressed in hepatoma. So, it is likely that anti-FGF2 antibody A00121-1 will work for ELISA with hepatoma.
Boster Scientific Support
Answered: 2018-02-27
Question
Is a blocking peptide available for product anti-FGF2 antibody (A00121-1)?
Verified Customer
Verified customer
Asked: 2017-12-21
Answer
We do provide the blocking peptide for product anti-FGF2 antibody (A00121-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-21
Question
I see that the anti-FGF2 antibody A00121-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-05
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-05
Question
Does A00121-1 anti-FGF2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Li
Verified customer
Asked: 2016-09-30
Answer
It shows on the product datasheet, A00121-1 anti-FGF2 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-09-30
Question
Do you have a BSA free version of anti-FGF2 antibody A00121-1 available?
J. Patel
Verified customer
Asked: 2016-02-17
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-FGF2 antibody A00121-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-17
Question
We purchased anti-FGF2 antibody for WB on hepatoma placenta in a previous project. I am using mouse, and We intend to use the antibody for ELISA next. I am interested in examining hepatoma placenta as well as cartilage tissue in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
D. Yang
Verified customer
Asked: 2015-04-28
Answer
I viewed the website and datasheets of our anti-FGF2 antibody and I see that A00121-1 has been validated on mouse in both WB and ELISA. Thus A00121-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-04-28
Question
I have attached the WB image, lot number and protocol we used for hepatoma using anti-FGF2 antibody A00121-1. Please let me know if you require anything else.
M. Miller
Verified customer
Asked: 2013-12-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-24
Question
My question regarding product A00121-1, anti-FGF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Anderson
Verified customer
Asked: 2013-02-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00121-1 anti-FGF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-02-22
