Product Info Summary
SKU: | A03130-1 |
---|---|
Size: | 80 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IHC-P, WB |
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Product info
Product Name
Anti-RECQL4 Antibody(C-term)
SKU/Catalog Number
A03130-1
Size
80 µl
Description
Boster Bio Anti-RECQL4 Antibody(C-term) (Catalog # A03130-1). Tested in IHC-P, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RECQL4 Antibody(C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03130-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RECQL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1134-1162 amino acids from the C-terminal region of human RECQL4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03130-1 is reactive to RECQL4 in Human, Mouse
Reconstitution
Calculated molecular weight
133067 Da
Background of RECQL4
The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. [provided by RefSeq].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03130-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:100
Validation Images & Assay Conditions
Click image to see more details
All lanes : Anti-RECQL4 Antibody (C-term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: U-251 MG whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: Mouse testis tissue lysate
Lane 6: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 133 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Immunohistochemical analysis of A03130-1 on paraffin-embedded Human tonsil tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For RECQL4 (Source: Uniprot.org, NCBI)
Gene Name
RECQL4
Full Name
ATP-dependent DNA helicase Q4
Weight
133067 Da
Superfamily
helicase family
Alternative Names
ATP-dependent DNA helicase Q4, DNA helicase, RecQ-like type 4, RecQ4, RTS, RecQ protein-like 4, RECQL4, RECQ4 RECQL4 RECQ4 RecQ like helicase 4 ATP-dependent DNA helicase Q4|DNA helicase, RecQ-like, type 4|RecQ helicase-like 4|RecQ protein-like 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RECQL4, check out the RECQL4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RECQL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RECQL4 Antibody(C-term) (A03130-1)
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