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Product Info Summary
| SKU: | RP1006 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Fibroblast growth factor 2 FGF2 Antibody Picoband®
View all FGF basic/FGF2/bFGF Antibodies
SKU/Catalog Number
RP1006
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fibroblast growth factor 2 FGF2 Antibody catalog # RP1006. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibroblast growth factor 2 FGF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1006)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FGF2 recombinant protein (Position: P143-S288).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1006 is reactive to FGF2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17-21 kDa
Calculated molecular weight
30770 MW
Background of FGF basic/FGF2/bFGF
FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1006 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human SKOV3 whole cell, human SiHa whole cell, human u87 whole cell
ICC/IF: SiHa cell
FCM: SiHa cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (RP1006).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SKOV3 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human u87 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # RP1006) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.
Click image to see more details
Figure 2. IF analysis of FGF2 using anti-FGF2 antibody (RP1006).
FGF2 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FGF2 Antibody (RP1006) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of SiHa cells using anti-FGF2 antibody (RP1006).
Overlay histogram showing SiHa cells stained with A04887-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGF2 Antibody (RP1006, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For FGF2 (Source: Uniprot.org, NCBI)
Gene Name
FGF2
Full Name
Fibroblast growth factor 2
Weight
30770 MW
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB; FGF2 BFGF, FGF-2, FGFB, HBGF-2 fibroblast growth factor 2 fibroblast growth factor 2|basic fibroblast growth factor bFGF|fibroblast growth factor 2 (basic)|heparin-binding growth factor 2|prostatropin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF2, check out the FGF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibroblast growth factor 2 FGF2 Antibody Picoband® (RP1006)
Hello CJ!
RP1006 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen X,Zhao H,Chen C,Li J,He J,Fu X,Zhao H.The HPA/SDC1 axis promotes invasion and metastasis of pancreatic cancer cells by activating EMT via FGF2 upregulation.Oncol Lett.2020 Jan;19(1):211-220.doi: 10.3892/ol.2019.11121.Epub 2019 Nov 19.PMID:31897132;PM
Species: Human
RP1006 usage in article: APP:IHC, SAMPLE:PANCREATIC TUMOR TISSUE, DILUTION:1:100
Effect of CPU-XT-008, a combretastatin A-4 analogue, on the proliferation, apoptosis and expression of vascular endothelial growth factor and basic fibroblast growth factor in human umbilical vein endothelial cells
Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction
Advanced glycation end-products stimulate basic fibroblast growth factor expression in cultured M?ller cells
Zhou B, Ma R, Si W, Li S, Xu Y, Tu X, Wang Q. Cancer Lett. 2013 Jun 10;333(2):159-69. Doi: 10.1016/J.Canlet.2013.01.028. Epub 2013 Jan 22. Microrna-503 Targets Fgf2 And Vegfa And Inhibits Tumor Angiogenesis And Growth.
Hu C, Zhang T, Ren B, Deng Z, Cai L, Lei J, Ping A. Med Sci Monit. 2015 Apr 27;21:1200-6. Doi: 10.12659/Msm.892939. Effect Of Vacuum-Assisted Closure Combined With Open Bone Grafting To Promote Rabbit Bone Graft Vascularization.
Wang J, Wang Z, Yao Y, Wu J, Tang X, Gu T, Li G. Tuberculosis (Edinb). 2015 Jul;95(4):505-14. Doi: 10.1016/J.Tube.2015.04.006. Epub 2015 May 9. The Fibroblast Growth Factor-2 Arrests Mycobacterium Avium Sp. Paratuberculosis Growth And Immunomodula...
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Customer Q&As
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16 Customer Q&As for Anti-Fibroblast growth factor 2 FGF2 Antibody Picoband®
Question
Is a blocking peptide available for product anti-FGF2 antibody (RP1006)?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
We do provide the blocking peptide for product anti-FGF2 antibody (RP1006). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-27
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-FGF2 antibody RP1006. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-16
Question
Do you have a BSA free version of anti-FGF2 antibody RP1006 available?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FGF2 antibody RP1006 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-25
Question
I was wanting to use your anti-FGF2 antibody for ELISA for human blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
As indicated on the product datasheet, RP1006 anti-FGF2 antibody has been validated for ELISA, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-24
Question
Does RP1006 anti-FGF2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
As indicated on the product datasheet, RP1006 anti-FGF2 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-13
Question
you antibody to test anti-FGF2 antibody RP1006 on human blood for research purposes, then I may be interested in using anti-FGF2 antibody RP1006 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
The products we sell, including anti-FGF2 antibody RP1006, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-25
Question
My colleagues were content with the WB result of your anti-FGF2 antibody. However we have been able to see positive staining in hepatoma secreted. nucleus. note=exported from cells using this antibody. Is that expected? Could you tell me where is FGF2 supposed to be expressed?
T. Singh
Verified customer
Asked: 2019-05-24
Answer
Based on literature, hepatoma does express FGF2. Generally FGF2 expresses in secreted. nucleus. note=exported from cells. Regarding which tissues have FGF2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 2435575
Hepatoma, Pubmed ID: 1721615, 2538817
Hepatoma, and Placenta, Pubmed ID: 3579930
Boster Scientific Support
Answered: 2019-05-24
Question
We are currently using anti-FGF2 antibody RP1006 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
The anti-FGF2 antibody (RP1006) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-19
Question
I see that the anti-FGF2 antibody RP1006 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-17
Question
We have tried in the past anti-FGF2 antibody for WB on cartilage tissue in the past. I am using human, and I plan to use the antibody for IHC next. We want examining cartilage tissue as well as hepatoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
N. Johnson
Verified customer
Asked: 2017-09-28
Answer
I have checked the website and datasheets of our anti-FGF2 antibody and it appears that RP1006 has been validated on human in both WB and IHC. Thus RP1006 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-28
Question
Here is the WB image, lot number and protocol we used for blood using anti-FGF2 antibody RP1006. Please let me know if you require anything else.
T. Brown
Verified customer
Asked: 2016-07-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-25
Question
Is this RP1006 anti-FGF2 antibody reactive to the isotypes of FGF2?
K. Li
Verified customer
Asked: 2015-11-02
Answer
The immunogen of RP1006 anti-FGF2 antibody is E.coli-derived human FGF2 recombinant protein(Position: P143-S288). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-11-02
Question
We have been able to see staining in human hepatoma. What should we do? Is anti-FGF2 antibody supposed to stain hepatoma positively?
A. Wu
Verified customer
Asked: 2015-10-28
Answer
From literature hepatoma does express FGF2. From Uniprot.org, FGF2 is expressed in cartilage tissue, hepatoma, blood, hepatoma placenta, among other tissues. Regarding which tissues have FGF2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 2435575
Hepatoma, Pubmed ID: 1721615, 2538817
Hepatoma, and Placenta, Pubmed ID: 3579930
Boster Scientific Support
Answered: 2015-10-28
Question
Would anti-FGF2 antibody RP1006 work for ELISA with blood?
L. Krishna
Verified customer
Asked: 2015-09-18
Answer
According to the expression profile of blood, FGF2 is highly expressed in blood. So, it is likely that anti-FGF2 antibody RP1006 will work for ELISA with blood.
Boster Scientific Support
Answered: 2015-09-18
Question
My question regarding product RP1006, anti-FGF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Edwards
Verified customer
Asked: 2015-01-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1006 anti-FGF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-01-12
Question
Does anti-FGF2 antibody RP1006 work on goat IHC with hepatoma?
S. Evans
Verified customer
Asked: 2013-05-15
Answer
Our lab technicians have not validated anti-FGF2 antibody RP1006 on goat. You can run a BLAST between goat and the immunogen sequence of anti-FGF2 antibody RP1006 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat hepatoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-05-15
