Anti-FGF2 Antibody

SKU RP1006
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications ELISA, IHC, WB

Overview

Product Name Anti-FGF2 Antibody
SKU/Catalog Number RP1006
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Fibroblast growth factor 2(FGF2) detection. Tested with WB, IHC-P, ELISA in Human.
Cite This Product Anti-FGF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1006)
Host Rabbit
Contents/Buffer Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Form Lyophilized
Immunogen E.coli-derived human FGF2 recombinant protein(Position: P143-S288).
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

Anti- FGF2 antibody, RP1006, Western blotting
All lanes: Anti FGF2(RP1006) at 0.5ug/ml
Lane 1: Recombinant Human FGF2 Protein 10ng
Lane 2: Recombinant Human FGF2 Protein 5ng
Lane 3: Recombinant Human FGF2 Protein 2.5ng
Predicted bind size:

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P09038
Gene Name FGF2
Protein Name Fibroblast growth factor 2
Tissue Specificity Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. .
Alternative Names Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB;
Subcellular Localization Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
Molecular Weight 30770 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. .
Research Areas Human

*You can search these to find other products in these research areas.
Background FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor(FGF) family. Fibroblast growth factors(FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

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In stock
Order Product
RP1006
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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Publications

Effect of CPU-XT-008, a combretastatin A-4 analogue, on the proliferation, apoptosis and expression of vascular endothelial growth factor and basic fibroblast growth factor in human umbilical vein endothelial cells
Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction
Advanced glycation end-products stimulate basic fibroblast growth factor expression in cultured M?ller cells
Zhou B, Ma R, Si W, Li S, Xu Y, Tu X, Wang Q. Cancer Lett. 2013 Jun 10;333(2):159-69. Doi: 10.1016/J.Canlet.2013.01.028. Epub 2013 Jan 22. Microrna-503 Targets Fgf2 And Vegfa And Inhibits Tumor Angiogenesis And Growth.
Hu C, Zhang T, Ren B, Deng Z, Cai L, Lei J, Ping A. Med Sci Monit. 2015 Apr 27;21:1200-6. Doi: 10.12659/Msm.892939. Effect Of Vacuum-Assisted Closure Combined With Open Bone Grafting To Promote Rabbit Bone Graft Vascularization.
Wang J, Wang Z, Yao Y, Wu J, Tang X, Gu T, Li G. Tuberculosis (Edinb). 2015 Jul;95(4):505-14. Doi: 10.1016/J.Tube.2015.04.006. Epub 2015 May 9. The Fibroblast Growth Factor-2 Arrests Mycobacterium Avium Sp. Paratuberculosis Growth And Immunomodula...

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
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