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Product Info Summary
| SKU: | EK1406 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human COMT ELISA Kit PicoKine®
SKU/Catalog Number
EK1406
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human COMT ELISA Kit PicoKine® (96 Tests). Quantitate Human COMT in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human COMT ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1406)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: G52-P271
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1406 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1406 is reactive to COMT in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1406 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of COMT
Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines. In the brain, COMT-dependent dopamine degradation is of particular importance in brain regions with low expression of the presynaptic dopamine transporter (DAT), such as the prefrontal cortex. This process is supposed to take place in postsynaptic neurons, as, in general, COMT is located intracellularly in the central nervous system (CNS).
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1406-CAP | Anti-Human COMT Pre-coated 96-well strip microplate | 1 |
| EK1406-ST | Human COMT Standard | 2 vials, 10 ng/tube |
| EK1406-DA | Human COMT Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.026 | 0.087 | 0.134 | 0.275 | 0.492 | 0.904 | 1.492 | 2.223 |
Data Example Images
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Human COMT PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect COMT, Dilution ratio of 1:1, concentration in serum and plasma is 100-214 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human COMT ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 71 | 245 | 923 | 65 | 220 | 946 |
| Standard deviation | 4.97 | 16.17 | 61.84 | 8.43 | 16.5 | 79.46 |
| CV (%) | 7% | 6.6% | 6.7% | 7.9% | 7.5% | 8.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of COMT in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 71 | 72 | 67 | 62 | 68 | 3.93 | 5.7% |
| Sample 2 | 245 | 245 | 279 | 270 | 259 | 15.08 | 5.8% |
| Sample 3 | 923 | 1061 | 1051 | 966 | 1000 | 57.89 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For COMT (Source: Uniprot.Org, NCBI)
Gene Name
COMT
Full Name
Catechol O-methyltransferase
Weight
30037 MW
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
Catechol O-methyltransferase;2.1.1.6;COMT; COMT HEL-S-98n catechol-O-methyltransferase catechol O-methyltransferase|epididymis secretory sperm binding protein Li 98n|testicular tissue protein Li 42
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on COMT, check out the COMT Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for COMT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human COMT ELISA Kit PicoKine® (EK1406)
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No publications found for EK1406
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human COMT ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I measured COMT level in cell lysates.
Verified Customer
Verified customer
Asked: 2020-01-11
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-01-11
Question
What buffer is recommended with cell lysate using the EK1406 kit?
Verified customer
Asked: 2019-12-23
Answer
The recommended buffer with cell lysate using the Human COMT ELISA Kit PicoKine® (EK1406) is IP Lysis Buffer AR0107.
Boster Scientific Support
Answered: 2019-12-27
Question
Was a baculovirus expression system used for the manufacturing process of EK1406?
Verified customer
Asked: 2019-12-01
Answer
The Human COMT ELISA Kit PicoKine™ (EK1406) does not use baculovirus expression system. However our ELISA kits that has the expression systems of sf9 and sf21 use baculovirrus expression system.
Boster Scientific Support
Answered: 2019-12-03
Question
Has EK1406 kit been tested on human feces samples?
Verified customer
Asked: 2019-08-22
Answer
Our lab has not tested the Human COMT ELISA Kit PicoKine EK1406 on human feces samples before. There is no guarantee that the ELISA kit will work for the samples since we do not have much experience in testing feces samples. It is suggested to make sure that the samples are liquid without any solid impurities prior to use if doing a pilot test.
Boster Scientific Support
Answered: 2019-08-23
Question
Q: Can COMT ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-04-11
Question
Q: if the enzyme conjugated COMT antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the COMT antigen?
Verified Customer
Verified customer
Asked: 2018-09-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-09-23
Question
Q: can I use citrate plasma as samples in Human COMT Picokine® ELISA Kit (Catalog # EK1406)?
W. Surve
Verified customer
Asked: 2018-02-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of COMT causing degradation of its protein structure. COMT may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the COMT ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-02-26
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Phillips
Verified customer
Asked: 2018-02-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-02-11
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
J. Zhang
Verified customer
Asked: 2017-06-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-06-10
Question
Q: how to thaw whole blood sample for COMT ELISA after freezing?
Verified Customer
Verified customer
Asked: 2015-11-02
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test COMT for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2015-11-02
