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Product Info Summary
| SKU: | PB9587 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FGF2 Antibody Picoband™
View all FGF basic/FGF2/bFGF Antibodies
SKU/Catalog Number
PB9587
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGF2 Antibody Picoband™ catalog # PB9587. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9587)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human FGF2, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9587 is reactive to FGF2 in Human, Rat
Applications
PB9587 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
18, 20 kDa
Calculated molecular weight
30770 MW
Background of FGF basic/FGF2/bFGF
FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (PB9587).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SKOV3 whole cell lysates,
Lane 3: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # PB9587) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 18, 20 kDa. The expected band size for FGF2 is at 18, 22, 30 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FGF2 (Source: Uniprot.org, NCBI)
Gene Name
FGF2
Full Name
Fibroblast growth factor 2
Weight
30770 MW
Superfamily
heparin-binding growth factors family
Alternative Names
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin FGF2 BFGF, FGF-2, FGFB, HBGF-2 fibroblast growth factor 2 fibroblast growth factor 2|basic fibroblast growth factor bFGF|fibroblast growth factor 2 (basic)|heparin-binding growth factor 2|prostatropin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF2, check out the FGF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGF2 Antibody Picoband™ (PB9587)
Hello CJ!
PB9587 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of CPU-XT-008, a combretastatin A-4 analogue, on the proliferation, apoptosis and expression of vascular endothelial growth factor and basic fibroblast growth factor in human umbilical vein endothelial cells
Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction
Advanced glycation end-products stimulate basic fibroblast growth factor expression in cultured M?ller cells
Zhou B, Ma R, Si W, Li S, Xu Y, Tu X, Wang Q. Cancer Lett. 2013 Jun 10;333(2):159-69. Doi: 10.1016/J.Canlet.2013.01.028. Epub 2013 Jan 22. Microrna-503 Targets Fgf2 And Vegfa And Inhibits Tumor Angiogenesis And Growth.
Hu C, Zhang T, Ren B, Deng Z, Cai L, Lei J, Ping A. Med Sci Monit. 2015 Apr 27;21:1200-6. Doi: 10.12659/Msm.892939. Effect Of Vacuum-Assisted Closure Combined With Open Bone Grafting To Promote Rabbit Bone Graft Vascularization.
Wang J, Wang Z, Yao Y, Wu J, Tang X, Gu T, Li G. Tuberculosis (Edinb). 2015 Jul;95(4):505-14. Doi: 10.1016/J.Tube.2015.04.006. Epub 2015 May 9. The Fibroblast Growth Factor-2 Arrests Mycobacterium Avium Sp. Paratuberculosis Growth And Immunomodula...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-FGF2 Antibody Picoband™
Question
I was wanting to use your anti-FGF2 antibody for WB for human cartilage tissue on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cartilage tissue identification?
A. Walker
Verified customer
Asked: 2019-11-22
Answer
It shows on the product datasheet, PB9587 anti-FGF2 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cartilage tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-22
Question
We are currently using anti-FGF2 antibody PB9587 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The anti-FGF2 antibody (PB9587) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-14
Question
What kind of lyses and sample buffer do you use to prepare the rat brain samples for the western blot? Keyword: protocol, reagents
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We use RIPA lysate solution(AR0105) and SDS-PAGE loading buffer(AR0131).
Boster Scientific Support
Answered: 2019-11-01
Question
Is this PB9587 anti-FGF2 antibody reactive to the isotypes of FGF2?
M. Moore
Verified customer
Asked: 2019-10-24
Answer
The immunogen of PB9587 anti-FGF2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human FGF2 (219-249aa KEDGRLLASKCVTDECFFFERLESNNYNTYR), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-24
Question
I see that the anti-FGF2 antibody PB9587 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cartilage tissue using anti-FGF2 antibody PB9587. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-12
Question
My boss were well pleased with the WB result of your anti-FGF2 antibody. However we have been able to see positive staining in hepatoma placenta secreted. nucleus. note=exported from cells using this antibody. Is that expected? Could you tell me where is FGF2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
From literature, hepatoma placenta does express FGF2. Generally FGF2 expresses in secreted. nucleus. note=exported from cells. Regarding which tissues have FGF2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 2435575
Hepatoma, Pubmed ID: 1721615, 2538817
Hepatoma, and Placenta, Pubmed ID: 3579930
Boster Scientific Support
Answered: 2019-07-26
Question
We want using your anti-FGF2 antibody for non-integrin membrane-ecm interactions studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
Thanks for your inquiry. This PB9587 anti-FGF2 antibody is tested on human placenta tissue, tissue lysate, rat spleen tissue, brain tissue, kidney tissue. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-15
Question
Is a blocking peptide available for product anti-FGF2 antibody (PB9587)?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We do provide the blocking peptide for product anti-FGF2 antibody (PB9587). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-28
Question
Please see the WB image, lot number and protocol we used for cartilage tissue using anti-FGF2 antibody PB9587. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-13
Question
Does PB9587 anti-FGF2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
As indicated on the product datasheet, PB9587 anti-FGF2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-07
Question
I have a question about product PB9587, anti-FGF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9587 anti-FGF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-20
Question
Would anti-FGF2 antibody PB9587 work for WB with cartilage tissue?
A. Banerjee
Verified customer
Asked: 2017-08-01
Answer
According to the expression profile of cartilage tissue, FGF2 is highly expressed in cartilage tissue. So, it is likely that anti-FGF2 antibody PB9587 will work for WB with cartilage tissue.
Boster Scientific Support
Answered: 2017-08-01
Question
We have observed staining in rat cartilage tissue. Do you have any suggestions? Is anti-FGF2 antibody supposed to stain cartilage tissue positively?
Verified Customer
Verified customer
Asked: 2017-07-12
Answer
From literature cartilage tissue does express FGF2. From Uniprot.org, FGF2 is expressed in cartilage tissue, hepatoma, blood, hepatoma placenta, among other tissues. Regarding which tissues have FGF2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 2435575
Hepatoma, Pubmed ID: 1721615, 2538817
Hepatoma, and Placenta, Pubmed ID: 3579930
Boster Scientific Support
Answered: 2017-07-12
Question
you antibody to test anti-FGF2 antibody PB9587 on human cartilage tissue for research purposes, then I may be interested in using anti-FGF2 antibody PB9587 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
The products we sell, including anti-FGF2 antibody PB9587, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-06
Question
Do you have a BSA free version of anti-FGF2 antibody PB9587 available?
T. Krishna
Verified customer
Asked: 2014-09-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FGF2 antibody PB9587 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-09-02
