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Product Info Summary
| SKU: | EK1766 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human TINAGL1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1766
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TINAGL1 ELISA Kit PicoKine® (96 Tests). Quantitate Human TINAGL1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TINAGL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1766)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A22-H467
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1766 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1766 is reactive to TINAGL1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK1766 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TINAGL1
Tubulointerstitial nephritis antigen-like is a protein that in humans is encoded by the TINAGL1 gene. It is mapped to 1p35.2. The protein encoded by this gene is similar in sequence to tubulointerstitial nephritis antigen, a secreted glycoprotein that is recognized by antibodies in some types of immune-related tubulointerstitial nephritis. Three transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1766-CAP | Anti-Human TINAGL1 Pre-coated 96-well strip microplate | 1 |
| EK1766-ST | Human TINAGL1 Standard | 2 vials, 10 ng/tube |
| EK1766-DA | Human TINAGL1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.045 | 0.137 | 0.182 | 0.295 | 0.496 | 0.897 | 1.463 | 2.025 |
Data Example Images
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Human TINAGL1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TINAGL1, Dilution ratio of 1:1, concentration in serum and plasma is around 500 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TINAGL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 43 | 216 | 672 | 52 | 225 | 857 |
| Standard deviation | 1.76 | 12.31 | 30.91 | 3.02 | 15.53 | 54.85 |
| CV (%) | 4.1% | 5.7% | 4.6% | 5.8% | 6.9% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TINAGL1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 43 | 43 | 44 | 50 | 45 | 2.91 | 6.4% |
| Sample 2 | 216 | 209 | 214 | 213 | 213 | 2.54 | 1.1% |
| Sample 3 | 672 | 709 | 636 | 661 | 669 | 26.27 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For TINAGL1 (Source: Uniprot.Org, NCBI)
Gene Name
TINAGL1
Full Name
Tubulointerstitial nephritis antigen-like
Weight
52.387kDa
Superfamily
peptidase C1 family
Alternative Names
androgen-regulated gene 1; ARG1; GIS5; Glucocorticoid-inducible protein 5; LCN7; LIECG3; lipocalin 7; OLRG2; OLRG-2; Oxidized LDL-responsive gene 2 protein; oxidized-LDL responsive gene 2; P3ECSL; TIN Ag-related protein; TINAGL; TINAGL1; TINAG-like 1; TINAGRP; TIN-ag-RP; tubulointerstitial nephritis antigen-like 1; tubulointerstitial nephritis antigen-like; Tubulointerstitial nephritis antigen-related protein TINAGL1 ARG1, LCN7, LIECG3, TINAGRP tubulointerstitial nephritis like 1 tubulointerstitial nephritis -like|OLRG-2|P3ECSL|TIN Ag-related protein|TIN-Ag-RP|TINAG-like 1|androgen-regulated gene 1|glucocorticoid-inducible protein 5|lipocalin 7|oxidized-LDL responsive gene 2|tubulointerstitial nephritis -related protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TINAGL1, check out the TINAGL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TINAGL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TINAGL1 ELISA Kit PicoKine® (EK1766)
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Customer Q&As
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11 Customer Q&As for Human TINAGL1 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for TINAGL1 ELISA kit? I performed your TINAGL1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TINAGL1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TINAGL1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TINAGL1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-02-10
Question
What is the Capture Host/Clonality for EK1766?
Verified customer
Asked: 2019-08-29
Answer
The Capture Host/Clonality for the Human TINAGL1 ELISA Kit PicoKine™ (EK1766) is sheep polyclonal.
Boster Scientific Support
Answered: 2019-08-30
Question
What is the Capture/Detection Purification for EK1766?
Verified customer
Asked: 2019-08-29
Answer
The Capture/Detection Purification used for the Human TINAGL1 ELISA Kit PicoKine™ (EK1766) is Affinity Purified.
Boster Scientific Support
Answered: 2019-08-30
Question
What is the Standard Source and Expression for EK1766?
Verified customer
Asked: 2019-08-29
Answer
The Standard Source for the Human TINAGL1 ELISA Kit PicoKine™ (EK1766) is Recombinant Protein. The Standard Expression is NSO Expression System.
Boster Scientific Support
Answered: 2019-08-30
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
L. Puri
Verified customer
Asked: 2019-08-26
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-08-26
Question
Q: can you recommend the dilution ratio of serum samples for detection of TINAGL1 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-11-14
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TINAGL1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-14
Question
Q: how do I analyze ELISA data? I have obtained TINAGL1 level in serum.
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-05-01
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-01-03
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-01-03
Question
Q: can I use heparin plasma as samples in Human TINAGL1 Picokine® ELISA Kit (Catalog # EK1766)?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TINAGL1 causing degradation of its protein structure. TINAGL1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TINAGL1 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-12-15
Question
Q: if the enzyme conjugated TINAGL1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TINAGL1 antigen?
P. Krishna
Verified customer
Asked: 2014-05-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-05-11
Question
Q: how to thaw whole blood sample for TINAGL1 ELISA after freezing?
W. Wilson
Verified customer
Asked: 2014-03-17
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TINAGL1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2014-03-17
