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Product Info Summary
| SKU: | PA2308 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-C5/C5a Antibody Picoband®
SKU/Catalog Number
PA2308
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-C5/C5a Antibody catalog # PA2308. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C5/C5a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2308)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat C5a, different from the related mouse sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2308 is reactive to C5 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
115 kDa
Calculated molecular weight
8981 MW
Background of C5
C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2308 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Liver Tissue, Mouse Liver Tissue, NIH Whole Cell, HEPA Whole Cell, PC12 Whole Cell
IHC: Mouse Spleen Tissue, Rat Liver Tissue, Rat Spleen Tissue
Validation Images & Assay Conditions
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Anti-C5/C5a antibody, PA2308, IHC(P)
IHC(P): Mouse Spleen Tissue
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Anti-C5/C5a antibody, PA2308, IHC(P)
IHC(P): Rat Liver Tissue
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Anti-C5/C5a antibody, PA2308, IHC(P)
IHC(P): Rat Spleen Tissue
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Anti-C5/C5a antibody, PA2308, All Western blotting
All lanes: Anti-C5a(PA2308) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Mouse Liver Tissue Lysate at 40ug
Lane 3: NIH Whole Cell Lysate at 40ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Lane 5: PC12 Whole Cell Lysate at 40ug
Predicted bind size: 115KD
Observed bind size: 115KD
Protein Target Info & Infographic
Gene/Protein Information For C5 (Source: Uniprot.org, NCBI)
Gene Name
C5
Full Name
Complement C5
Weight
8981 MW
Alternative Names
Complement C5;C5a anaphylatoxin;C5; C5 C5Da, C5b, CPAMD4, ECLZB, C5 complement C5 complement C5|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4|C5a anaphylatoxin|anaphylatoxin C5a analog|complement component 5|prepro-C5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on C5, check out the C5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for C5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C5/C5a Antibody Picoband® (PA2308)
Hello CJ!
PA2308 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
C5a aggravates dysfunction of the articular cartilage and synovial fluid in rats with knee joint immobilization
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Anti-C5/C5a Antibody Picoband®
Question
Can you let me know what antigen retrieval procedure was used for PA2308?
Verified customer
Asked: 2022-06-20
Answer
Please see the antigen retrieval procedure for the Anti-C5/C5a Antibody (PA2308), Heat-induced epitope retrieval: Immerse the slides in antigen retrieval buffer (EDTA buffer, pH 8.0). Microwave the solution at Medium-high power for 8 minutes. Cool the slides for 5 minutes. Then microwave the solution at High power for 4 minutes. Cool the slides to room temperature.
Boster Scientific Support
Answered: 2022-06-21
Question
I have attached the WB image, lot number and protocol we used for plasma using anti-C5/C5a antibody PA2308. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-31
Question
Is there a BSA free version of anti-C5/C5a antibody PA2308 available?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-C5/C5a antibody PA2308 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-20
Question
Would PA2308 anti-C5/C5a antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
You can see on the product datasheet, PA2308 anti-C5/C5a antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-C5/C5a antibody PA2308. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-18
Question
I see that the anti-C5/C5a antibody PA2308 works with WB, what is the protocol used to produce the result images on the product page?
F. Mitchell
Verified customer
Asked: 2019-07-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-29
Question
Is a blocking peptide available for product anti-C5/C5a antibody (PA2308)?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
We do provide the blocking peptide for product anti-C5/C5a antibody (PA2308). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-23
Question
Does anti-C5/C5a antibody PA2308 work for WB with plasma?
Verified Customer
Verified customer
Asked: 2017-09-20
Answer
According to the expression profile of plasma, C5 is highly expressed in plasma. So, it is likely that anti-C5/C5a antibody PA2308 will work for WB with plasma.
Boster Scientific Support
Answered: 2017-09-20
