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Product Info Summary
| SKU: | A01060 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Chk1/CHEK1 Antibody Picoband®
SKU/Catalog Number
A01060
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Chk1/CHEK1 Antibody Picoband® catalog # A01060. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Chk1/CHEK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01060)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Chk1 recombinant protein (Position: M1-Q210). Human Chk1 shares 96.7% and 97.6% amino acid (aa) sequence identity with mouse and rat Chk1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01060 is reactive to CHEK1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
54 kDa
Calculated molecular weight
54434 MW
Background of Chk1
CHEK1, Cell cycle checkpoint kinase, is an enzyme that in humans is encoded by the CHEK1 gene. By fluorescence in situ hybridization, the human CHEK1 gene is mapped to 11q24, near the ATM gene at 11q23. CHEK1 is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Furthermore, CHEK1 acts to integrate signals from ATM and ATR, and is involved in monitoring meiotic recombination, a process that involves programmed DNA breaks.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01060 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human 293T whole cell, human K562 whole cell
FCM: K562 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Chk1/CHEK1 using anti-Chk1/CHEK1 antibody (A01060).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chk1/CHEK1 antigen affinity purified polyclonal antibody (Catalog # A01060) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chk1/CHEK1 at approximately 54 kDa. The expected band size for Chk1/CHEK1 is at 54 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of K562 cells using anti-Chk1/CHEK1 antibody (A01060).
Overlay histogram showing K562 cells stained with A01060 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Chk1/CHEK1 Antibody (A01060, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CHEK1 (Source: Uniprot.org, NCBI)
Gene Name
CHEK1
Full Name
Serine/threonine-protein kinase Chk1
Weight
54434 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase Chk1;2.7.11.1;CHK1 checkpoint homolog;Cell cycle checkpoint kinase;Checkpoint kinase-1;CHEK1;CHK1; CHEK1 CHK1 checkpoint kinase 1 serine/threonine-protein kinase Chk1|CHK1 checkpoint homolog|Checkpoint, S. pombe, homolog of, 1|Chk1-S|cell cycle checkpoint kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHEK1, check out the CHEK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHEK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Chk1/CHEK1 Antibody Picoband® (A01060)
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No publications found for A01060
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Customer Q&As
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15 Customer Q&As for Anti-Chk1/CHEK1 Antibody Picoband®
Question
Is this A01060 anti-Chk1/CHEK1 antibody reactive to the isotypes of CHEK1?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
The immunogen of A01060 anti-Chk1/CHEK1 antibody is E.coli-derived human Chk1 recombinant protein (Position: M1-Q210). Human Chk1 shares 96.7% and 97.6% amino acid (aa) sequence identity with mouse and rat Chk1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-12
Question
I was wanting to use to test anti-Chk1/CHEK1 antibody A01060 on human secondary oocyte for research purposes, then I may be interested in using anti-Chk1/CHEK1 antibody A01060 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
W. Li
Verified customer
Asked: 2019-11-29
Answer
The products we sell, including anti-Chk1/CHEK1 antibody A01060, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-29
Question
We are currently using anti-Chk1/CHEK1 antibody A01060 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The anti-Chk1/CHEK1 antibody (A01060) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-30
Question
Do you have a BSA free version of anti-Chk1/CHEK1 antibody A01060 available?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Chk1/CHEK1 antibody A01060 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-01
Question
Our lab were well pleased with the WB result of your anti-Chk1/CHEK1 antibody. However we have seen positive staining in cervix carcinoma erythroleukemia nucleus. cytoplasm. cytoplasm using this antibody. Is that expected? Could you tell me where is CHEK1 supposed to be expressed?
P. Miller
Verified customer
Asked: 2019-04-22
Answer
From literature, cervix carcinoma erythroleukemia does express CHEK1. Generally CHEK1 expresses in nucleus. cytoplasm. cytoplasm, cytoskeleton,. Regarding which tissues have CHEK1 expression, here are a few articles citing expression in various tissues:
Bone marrow, and Muscle, Pubmed ID: 15489334
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal thymus, Pubmed ID: 22184239
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2019-04-22
Question
I see that the anti-Chk1/CHEK1 antibody A01060 works with WB, what is the protocol used to produce the result images on the product page?
L. Parker
Verified customer
Asked: 2019-04-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-17
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for secondary oocyte using anti-Chk1/CHEK1 antibody A01060. Let me know if you need anything else.
N. Roberts
Verified customer
Asked: 2018-08-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-16
Question
I was wanting to use your anti-Chk1/CHEK1 antibody for WB for human secondary oocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human secondary oocyte identification?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
You can see on the product datasheet, A01060 anti-Chk1/CHEK1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human secondary oocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-21
Question
We have observed staining in human embryonic kidney. What should we do? Is anti-Chk1/CHEK1 antibody supposed to stain embryonic kidney positively?
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
From what I have seen in literature embryonic kidney does express CHEK1. From what I have seen in Uniprot.org, CHEK1 is expressed in secondary oocyte, fetal thymus, brain testis, bone marrow muscle, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CHEK1 expression, here are a few articles citing expression in various tissues:
Bone marrow, and Muscle, Pubmed ID: 15489334
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal thymus, Pubmed ID: 22184239
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2018-01-30
Question
See below the WB image, lot number and protocol we used for secondary oocyte using anti-Chk1/CHEK1 antibody A01060. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-29
Question
Will A01060 anti-Chk1/CHEK1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
W. Jackson
Verified customer
Asked: 2017-11-27
Answer
You can see on the product datasheet, A01060 anti-Chk1/CHEK1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-27
Question
I have a question about product A01060, anti-Chk1/CHEK1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01060 anti-Chk1/CHEK1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-07
Question
Is a blocking peptide available for product anti-Chk1/CHEK1 antibody (A01060)?
P. Baker
Verified customer
Asked: 2016-07-06
Answer
We do provide the blocking peptide for product anti-Chk1/CHEK1 antibody (A01060). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-07-06
Question
My question regards using your anti-Chk1/CHEK1 antibody for chk1/chk2(cds1) mediated inactivation of cyclin b:cdk1 complex studies. Has this antibody been tested with western blotting on sw620 whole cell lysates? We would like to see some validation images before ordering.
F. Miller
Verified customer
Asked: 2016-06-09
Answer
I appreciate your inquiry. This A01060 anti-Chk1/CHEK1 antibody is validated on sw620 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-06-09
Question
Will anti-Chk1/CHEK1 antibody A01060 work for WB with secondary oocyte?
C. Bhatt
Verified customer
Asked: 2016-02-17
Answer
According to the expression profile of secondary oocyte, CHEK1 is highly expressed in secondary oocyte. So, it is likely that anti-Chk1/CHEK1 antibody A01060 will work for WB with secondary oocyte.
Boster Scientific Support
Answered: 2016-02-17
