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Product Info Summary
| SKU: | M01060 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Chk1 CHEK1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01060
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Chk1 CHEK1 Rabbit Monoclonal Antibody catalog # M01060. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Chk1 CHEK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01060)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EIB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Chk1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01060 is reactive to CHEK1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
90 kDa
Calculated molecular weight
54434 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01060 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Chk1 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CHEK1 (Source: Uniprot.org, NCBI)
Gene Name
CHEK1
Full Name
Serine/threonine-protein kinase Chk1
Weight
54434 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase Chk1;2.7.11.1;CHK1 checkpoint homolog;Cell cycle checkpoint kinase;Checkpoint kinase-1;CHEK1;CHK1; CHEK1 CHK1 checkpoint kinase 1 serine/threonine-protein kinase Chk1|CHK1 checkpoint homolog|Checkpoint, S. pombe, homolog of, 1|Chk1-S|cell cycle checkpoint kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHEK1, check out the CHEK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHEK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Chk1 CHEK1 Rabbit Monoclonal Antibody (M01060)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Chk1 CHEK1 Rabbit Monoclonal Antibody
Question
I am interested in using your anti-Chk1 Rabbit Monoclonal antibody for intracellular signal transduction studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
Thanks for your inquiry. This M01060 anti-Chk1 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-27
Question
Would anti-Chk1 Rabbit Monoclonal antibody M01060 work for IF with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
According to the expression profile of cervix carcinoma erythroleukemia, CHEK1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Chk1 Rabbit Monoclonal antibody M01060 will work for IF with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-11-26
Question
I have a question about product M01060, anti-Chk1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01060 anti-Chk1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-28
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Chk1 Rabbit Monoclonal antibody M01060. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-14
Question
Is a blocking peptide available for product anti-Chk1 Rabbit Monoclonal antibody (M01060)?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
We do provide the blocking peptide for product anti-Chk1 Rabbit Monoclonal antibody (M01060). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-24
Question
I see that the anti-Chk1 Rabbit Monoclonal antibody M01060 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-29
Question
We have been able to see staining in human cervix carcinoma. Do you have any suggestions? Is anti-Chk1 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
Based on literature cervix carcinoma does express CHEK1. Based on Uniprot.org, CHEK1 is expressed in secondary oocyte, fetal thymus, brain testis, bone marrow muscle, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CHEK1 expression, here are a few articles citing expression in various tissues:
Bone marrow, and Muscle, Pubmed ID: 15489334
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal thymus, Pubmed ID: 22184239
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2019-07-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Chk1 Rabbit Monoclonal antibody M01060. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-17
Question
Do you have a BSA free version of anti-Chk1 Rabbit Monoclonal antibody M01060 available?
S. Gonzalez
Verified customer
Asked: 2018-12-27
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Chk1 Rabbit Monoclonal antibody M01060 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-27
Question
I was wanting to use your anti-Chk1 Rabbit Monoclonal antibody for IF for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
As indicated on the product datasheet, M01060 anti-Chk1 Rabbit Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-25
Question
Is this M01060 anti-Chk1 Rabbit Monoclonal antibody reactive to the isotypes of CHEK1?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
The immunogen of M01060 anti-Chk1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Chk1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-19
Question
We ordered your anti-Chk1 Rabbit Monoclonal antibody for WB on secondary oocyte a few years ago. I am using human, and We intend to use the antibody for IF next. you antibody examining secondary oocyte as well as brain testis in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
I have checked the website and datasheets of our anti-Chk1 Rabbit Monoclonal antibody and it appears that M01060 has been tested on human in both WB and IF. Thus M01060 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-27
Question
We are currently using anti-Chk1 Rabbit Monoclonal antibody M01060 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
T. Jackson
Verified customer
Asked: 2017-06-27
Answer
The anti-Chk1 Rabbit Monoclonal antibody (M01060) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-27
Question
We were happy with the WB result of your anti-Chk1 Rabbit Monoclonal antibody. However we have observed positive staining in leukemic t-cell cytoskeleton, using this antibody. Is that expected? Could you tell me where is CHEK1 supposed to be expressed?
E. Krishna
Verified customer
Asked: 2017-05-05
Answer
Based on literature, leukemic t-cell does express CHEK1. Generally CHEK1 expresses in nucleus. cytoplasm. cytoplasm, cytoskeleton,. Regarding which tissues have CHEK1 expression, here are a few articles citing expression in various tissues:
Bone marrow, and Muscle, Pubmed ID: 15489334
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal thymus, Pubmed ID: 22184239
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2017-05-05
Question
My question regards to test anti-Chk1 Rabbit Monoclonal antibody M01060 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Chk1 Rabbit Monoclonal antibody M01060 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Miller
Verified customer
Asked: 2014-03-27
Answer
The products we sell, including anti-Chk1 Rabbit Monoclonal antibody M01060, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-03-27
Question
Will M01060 anti-Chk1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Kulkarni
Verified customer
Asked: 2013-02-11
Answer
You can see on the product datasheet, M01060 anti-Chk1 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-02-11
