Click image to see more details
Product Info Summary
| SKU: | M01060-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-Chk1 CHEK1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01060-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Chk1 CHEK1 Rabbit Monoclonal Antibody catalog # M01060-1. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Chk1 CHEK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01060-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAF-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Chk1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01060-1 is reactive to CHEK1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
54 kDa
Calculated molecular weight
54434 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01060-1 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:30
Positive Control
WB: human K562 whole cell, human CACO-2 whole cell, human Jurkat whole cell, human 293T whole cell, rat PC-12 whole cell, mouse thymus tissue, mouse SP2/0 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Chk1 using anti-Chk1 antibody (M01060-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse thymus tissue lysates,
Lane 7: mouse SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chk1 antigen affinity purified monoclonal antibody (Catalog # M01060-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chk1 at approximately 54 kDa. The expected band size for Chk1 is at 54 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CHEK1 (Source: Uniprot.org, NCBI)
Gene Name
CHEK1
Full Name
Serine/threonine-protein kinase Chk1
Weight
54434 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase Chk1;2.7.11.1;CHK1 checkpoint homolog;Cell cycle checkpoint kinase;Checkpoint kinase-1;CHEK1;CHK1; CHEK1 CHK1 checkpoint kinase 1 serine/threonine-protein kinase Chk1|CHK1 checkpoint homolog|Checkpoint, S. pombe, homolog of, 1|Chk1-S|cell cycle checkpoint kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHEK1, check out the CHEK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHEK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Chk1 CHEK1 Rabbit Monoclonal Antibody (M01060-1)
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Customer Q&As
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17 Customer Q&As for Anti-Chk1 CHEK1 Rabbit Monoclonal Antibody
Question
Does anti-Chk1 Rabbit Monoclonal antibody M01060-1 work for IP with fetal thymus?
T. Collins
Verified customer
Asked: 2020-01-29
Answer
According to the expression profile of fetal thymus, CHEK1 is highly expressed in fetal thymus. So, it is likely that anti-Chk1 Rabbit Monoclonal antibody M01060-1 will work for IP with fetal thymus.
Boster Scientific Support
Answered: 2020-01-29
Question
Do you have a BSA free version of anti-Chk1 Rabbit Monoclonal antibody M01060-1 available?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Chk1 Rabbit Monoclonal antibody M01060-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-27
Question
We have been able to see staining in rat cervix carcinoma. Are there any suggestions? Is anti-Chk1 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
From what I have seen in literature cervix carcinoma does express CHEK1. From what I have seen in Uniprot.org, CHEK1 is expressed in secondary oocyte, fetal thymus, brain testis, bone marrow muscle, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CHEK1 expression, here are a few articles citing expression in various tissues:
Bone marrow, and Muscle, Pubmed ID: 15489334
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal thymus, Pubmed ID: 22184239
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2020-01-23
Question
I see that the anti-Chk1 Rabbit Monoclonal antibody M01060-1 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-13
Question
Is this M01060-1 anti-Chk1 Rabbit Monoclonal antibody reactive to the isotypes of CHEK1?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The immunogen of M01060-1 anti-Chk1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Chk1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal thymus using anti-Chk1 Rabbit Monoclonal antibody M01060-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-20
Question
Will M01060-1 anti-Chk1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
It shows on the product datasheet, M01060-1 anti-Chk1 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-20
Question
Our lab want to know about using your anti-Chk1 Rabbit Monoclonal antibody for regulation of signal transduction by p53 class mediator studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Thank you for your inquiry. This M01060-1 anti-Chk1 Rabbit Monoclonal antibody is tested on k562 cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-17
Question
We are currently using anti-Chk1 Rabbit Monoclonal antibody M01060-1 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
The anti-Chk1 Rabbit Monoclonal antibody (M01060-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-06
Question
I was wanting to use your anti-Chk1 Rabbit Monoclonal antibody for IP for human fetal thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human fetal thymus identification?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
It shows on the product datasheet, M01060-1 anti-Chk1 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-26
Question
My question regarding product M01060-1, anti-Chk1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01060-1 anti-Chk1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-25
Question
Is a blocking peptide available for product anti-Chk1 Rabbit Monoclonal antibody (M01060-1)?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
We do provide the blocking peptide for product anti-Chk1 Rabbit Monoclonal antibody (M01060-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-14
Question
Here is the WB image, lot number and protocol we used for fetal thymus using anti-Chk1 Rabbit Monoclonal antibody M01060-1. Please let me know if you require anything else.
N. Collins
Verified customer
Asked: 2018-12-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-21
Question
We ordered your anti-Chk1 Rabbit Monoclonal antibody for IF on cervix carcinoma erythroleukemia a few years ago. I am using mouse, and We are going to use the antibody for IP next. My lab would like examining cervix carcinoma erythroleukemia as well as brain testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
I viewed the website and datasheets of our anti-Chk1 Rabbit Monoclonal antibody and it seems that M01060-1 has been validated on mouse in both IF and IP. Thus M01060-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-07
Question
We need to test anti-Chk1 Rabbit Monoclonal antibody M01060-1 on human fetal thymus for research purposes, then I may be interested in using anti-Chk1 Rabbit Monoclonal antibody M01060-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
The products we sell, including anti-Chk1 Rabbit Monoclonal antibody M01060-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-22
Question
My team were satisfied with the WB result of your anti-Chk1 Rabbit Monoclonal antibody. However we have observed positive staining in bone marrow muscle nucleus. cytoplasm. cytoplasm using this antibody. Is that expected? Could you tell me where is CHEK1 supposed to be expressed?
A. Lewis
Verified customer
Asked: 2014-12-17
Answer
Based on literature, bone marrow muscle does express CHEK1. Generally CHEK1 expresses in nucleus. cytoplasm. cytoplasm, cytoskeleton,. Regarding which tissues have CHEK1 expression, here are a few articles citing expression in various tissues:
Bone marrow, and Muscle, Pubmed ID: 15489334
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal thymus, Pubmed ID: 22184239
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2014-12-17
Question
Would anti-Chk1 Rabbit Monoclonal antibody M01060-1 work on feline IP with cervix carcinoma erythroleukemia?
K. Parker
Verified customer
Asked: 2014-11-06
Answer
Our lab technicians have not tested anti-Chk1 Rabbit Monoclonal antibody M01060-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Chk1 Rabbit Monoclonal antibody M01060-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline cervix carcinoma erythroleukemia in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-06
