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Product Info Summary
| SKU: | A01617-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CTNNA1 DyLight® 488 conjugated Antibody
View all Catenin alpha 1 Antibodies
SKU/Catalog Number
A01617-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CTNNA1 DyLight® 488 conjugated Antibody catalog # A01617-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CTNNA1 DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01617-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292). Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01617-Dyl488 is reactive to CTNNA1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
100.071kDa
Background of Catenin alpha 1
CTNNA1, also known as Catenin alpha-1 or Catenin (cadherin-associated protein), alpha 1, is a protein that in humans is encoded by the CTNNA1 gene. It is mapped to 5q31.2. When surface epithelium CTNNA1 was ablated, hair follicle development was blocked and epidermal morphogenesis was dramatically affected, with defects in adherens junction formation, intercellular adhesion, and epithelial polarity. In vitro, CTNNA1 null keratinocytes were poorly contact inhibited and grew rapidly. These differences were not dependent upon intercellular adhesion and were in marked contrast to keratinocytes conditionally null for another essential intercellular adhesion protein, desmoplakin Knockout keratinocytes exhibited sustained activation of the Ras-MAPK cascade due to aberrations in growth factor responses. It is concluded that features of precancerous lesions often attributed to defects in cell cycle regulatory genes can be generated by compromising the function of CTNNA1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01617-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
FCM: Hela cell
Validation Images & Assay Conditions
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1. Flow Cytometry analysis of Hela cells using anti-Human CTNNA1 antibody (A01617-Dyl488).
Overlay histogram showing Hela cells stained with A01617-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human CTNNA1 Antibody (A01617-Dyl488,1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CTNNA1 (Source: Uniprot.org, NCBI)
Gene Name
CTNNA1
Full Name
Catenin alpha-1
Weight
100.071kDa
Superfamily
vinculin/alpha-catenin family
Alternative Names
Catenin alpha-1; Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13; CTNNA1 CTNNA1 CAP102, MDPT2 catenin alpha 1 catenin alpha-1|alpha-E-catenin|catenin (cadherin-associated protein), alpha 1, 102kDa|epididymis secretory sperm binding protein|renal carcinoma NY-REN-13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTNNA1, check out the CTNNA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTNNA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CTNNA1 DyLight® 488 conjugated Antibody (A01617-Dyl488)
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