Anti-Human CTNNA1 DyLight® 488 conjugated Antibody

Product Name

Anti-Human CTNNA1 DyLight® 488 conjugated Antibody

View all Catenin alpha 1 Antibodies

SKU/Catalog Number

A01617-Dyl488

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human CTNNA1 DyLight® 488 conjugated Antibody catalog # A01617-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human CTNNA1 DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01617-Dyl488)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292). Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01617-Dyl488 is reactive to CTNNA1 in Human

Applications

A01617-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

Background of Catenin alpha 1

CTNNA1, also known as Catenin alpha-1 or Catenin (cadherin-associated protein), alpha 1, is a protein that in humans is encoded by the CTNNA1 gene. It is mapped to 5q31.2. When surface epithelium CTNNA1 was ablated, hair follicle development was blocked and epidermal morphogenesis was dramatically affected, with defects in adherens junction formation, intercellular adhesion, and epithelial polarity. In vitro, CTNNA1 null keratinocytes were poorly contact inhibited and grew rapidly. These differences were not dependent upon intercellular adhesion and were in marked contrast to keratinocytes conditionally null for another essential intercellular adhesion protein, desmoplakin Knockout keratinocytes exhibited sustained activation of the Ras-MAPK cascade due to aberrations in growth factor responses. It is concluded that features of precancerous lesions often attributed to defects in cell cycle regulatory genes can be generated by compromising the function of CTNNA1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry analysis of Hela cells using anti-Human CTNNA1 antibody (A01617-Dyl488).
Overlay histogram showing Hela cells stained with A01617-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human CTNNA1 Antibody (A01617-Dyl488,1μg/1x10⁶ cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CTNNA1 (Source: Uniprot.org, NCBI)

Alternative Names

Alpha E-catenin; alpha-catenin; alpha-E-catenin; alphaE-catenin; cadherin-associated protein; CAP102; catenin (cadherin-associated protein), alpha 1 (102kD); catenin (cadherin-associated protein), alpha 1, 102kDa; catenin alpha-1; FLJ36832; FLJ52416; Renal carcinoma antigen NY-REN-13 CTNNA1 CAP102, MDPT2 catenin alpha 1 catenin alpha-1|alpha-E-catenin|catenin (cadherin-associated protein), alpha 1, 102kDa|epididymis secretory sperm binding protein|renal carcinoma antigen NY-REN-13

For more info on CTNNA1, check out the CTNNA1 Infographic

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In this infographic, you will see the following information for CTNNA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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