Key=store_id Key=entity_id, value=1737 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=PA2157 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2014-05-08 15:19:26 Key=updated_at, value=2024-10-19 05:03:18 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-EIF2S2 Antibody Picoband® Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=Yes Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1575 Key=yoast_content_score, value=30 Key=badge, value=free-antibody-validation Key=price, value=370.0000 Key=weight, value=1.0000 Key=meta_title, value=Anti-EIF2S2 Antibody PA2157 Key=meta_description, value=Boster Bio Anti-EIF2S2 Antibody catalog # PA2157. Tested in WB applications. This antibody reacts with Human. Supplied as 100μg/vial in Lyophilized form antibody. Key=image, value=/p/a/pa2157-mag-primary-antibodies-wb-testing-1.jpg Key=small_image, value=/p/a/pa2157-mag-primary-antibodies-wb-testing-1.jpg Key=thumbnail, value=/p/a/pa2157-mag-primary-antibodies-wb-testing-1.jpg Key=url_key, value=anti-eif2s2-antibody-pa2157-boster Key=url_path, value=anti-eif2s2-antibody-pa2157-boster Key=options_container, value=Product Info Column Key=image_label, value=Figure 1. Western blot analysis of EIF2S2 using anti-EIF2S2 antibody (PA2157).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sam Key=small_image_label, value=Figure 1. Western blot analysis of EIF2S2 using anti-EIF2S2 antibody (PA2157).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sam Key=thumbnail_label, value=Figure 1. Western blot analysis of EIF2S2 using anti-EIF2S2 antibody (PA2157).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sam Key=msrp_display_actual_price_type, value=Use config Key=gift_message_available, value=No Key=size, value=100 μg/vial Key=protein_name, value=Eukaryotic translation initiation factor 2 subunit 2 Key=gene_full_name, value=Eukaryotic translation initiation factor 2 subunit 2 Key=uniprot_id, value=P20042 Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Key=sensitivity Key=applications, value=WB Key=reactivity, value=Human Key=form, value=Lyophilized Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. Key=cross_reactivity, value=No cross-reactivity with other proteins Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Key=predicted_reactivity Key=source_company, value=Boster PA2157 Key=isotype, value=Rabbit IgG Key=molecular_weight, value=38388 MW Key=sample_size_available, value=30ug for $99, contact us for details Key=related_product_skus, value=B0001-5,AR1156,AR0146 Key=product_category, value=Primary Antibodies Key=research_category, value=DNA/RNA, Epigenetics and Nuclear Signaling, Translation Key=clonality, value=Polyclonal Key=swatch_image, value=/e/k/ek1200.png Key=rating_value, value=96 Key=review_count, value=3 Key=phospho_site Key=mp_exclude_sitemap, value=Yes Key=yoast_focus_keyword, value=CD68 antibody Key=yoast_facebook_image, value=/p/a/pa1787-2-hexa-primary-antibodies-wb-testing-1_1.jpg Key=yoast_twitter_image, value=/p/a/pa1787-2-hexa-primary-antibodies-wb-testing-1_1.jpg Key=cj_related_products, value=Germ Cell Proliferation:EK0303|EK0301|EK0301-BV|EK0301-PO|EK0301-PR^Testicular Germ Cell Tumor:A02198 Key=out_of_stock_until_date, value=Aug 24, 2020 Key=description, value=Boster Bio Anti-EIF2S2 Antibody catalog # PA2157. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=short_description, value=Boster Bio Anti-EIF2S2 Antibody catalog # PA2157. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=meta_keyword, value=Anti-EIF2S2 Antibody Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Key=background, value=Eukaryotic translation initiation factor 2 subunit 2, also called EIF2S2 is a protein that in humans is encoded by the EIF2S2 gene. This gene belongs to the EIF-2-beta family. EIF2S2 is mapped to 20q11.2. Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. Key=gene_name, value=EIF2S2 Key=synonyms, value=Eukaryotic translation initiation factor 2 subunit 2;Eukaryotic translation initiation factor 2 subunit beta;eIF-2-beta;EIF2S2;EIF2B; Key=application_details, value=Western blot, 0.1-0.5μg/ml, Human
Key=subcellular_localization, value= Nucleus. Key=sequence_similarities, value=Belongs to the E2F/DP family. Key=tissue_specificity, value= Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Key=protein_function, value= eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Key=application_notes, value=Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 133133|sc 133209|sc 136972|sc 166536|sc 28851|sc 31886|sc 31888|sc 9978 Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of human EIF2S2, identical to the related mouse and rat sequences. Key=purification, value=Immunogen affinity purified. Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Key=custom_attribute_1, value=38 kDa Key=custom_attribute_2, value=WB: human HepG2 whole cell, human 293T whole cell, human Hela whole cell Key=custom_attribute_3, value=BA3422 Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-EIF2S2 Antibody Picoband®
EIF2 beta antibody
Boster Bio Anti-EIF2S2 Antibody catalog # PA2157. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Boster Bio Anti-EIF2S2 Antibody catalog # PA2157. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF2S2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2157)
A synthetic peptide corresponding to a sequence at the C-terminus of human EIF2S2, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2157 is reactive to EIF2S2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
38388 MW
Background of EIF2 beta
Eukaryotic translation initiation factor 2 subunit 2, also called EIF2S2 is a protein that in humans is encoded by the EIF2S2 gene. This gene belongs to the EIF-2-beta family. EIF2S2 is mapped to 20q11.2. Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human HepG2 whole cell, human 293T whole cell, human Hela whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of EIF2S2 using anti-EIF2S2 antibody (PA2157).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S2 antigen affinity purified polyclonal antibody (Catalog # PA2157) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2S2 at approximately 38 kDa. The expected band size for EIF2S2 is at 38 kDa.
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on EIF2S2, check out the EIF2S2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF2S2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
1 Customer Q&As for Anti-EIF2S2 Antibody Picoband®
Question
We are currently using anti-EIF2S2 antibody PA2157 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
The anti-EIF2S2 antibody (PA2157) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.