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Key=meta_description, value=Boster Bio Anti-EIF2S2 Antibody catalog # PA2157. Tested in WB applications. This antibody reacts with Human. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Figure 1. Western blot analysis of EIF2S2 using anti-EIF2S2 antibody (PA2157).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sam
Key=small_image_label, value=Figure 1. Western blot analysis of EIF2S2 using anti-EIF2S2 antibody (PA2157).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sam
Key=thumbnail_label, value=Figure 1. Western blot analysis of EIF2S2 using anti-EIF2S2 antibody (PA2157).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sam
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Key=size, value=100 μg/vial
Key=protein_name, value=Eukaryotic translation initiation factor 2 subunit 2
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Key=uniprot_id, value=P20042
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=sensitivity
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Key=reactivity, value=Human
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Key=predicted_reactivity
Key=source_company, value=Boster PA2157
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=38388 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=DNA/RNA, Epigenetics and Nuclear Signaling, Translation
Key=clonality, value=Polyclonal
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Key=out_of_stock_until_date, value=Aug 24, 2020
Key=description, value=Boster Bio Anti-EIF2S2 Antibody catalog # PA2157. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-EIF2S2 Antibody catalog # PA2157. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-EIF2S2 Antibody
Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Key=background, value=Eukaryotic translation initiation factor 2 subunit 2, also called EIF2S2 is a protein that in humans is encoded by the EIF2S2 gene. This gene belongs to the EIF-2-beta family. EIF2S2 is mapped to 20q11.2. Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation.
Key=gene_name, value=EIF2S2
Key=synonyms, value=Eukaryotic translation initiation factor 2 subunit 2;Eukaryotic translation initiation factor 2 subunit beta;eIF-2-beta;EIF2S2;EIF2B;
Key=application_details, value=Western blot, 0.1-0.5μg/ml, Human

Key=subcellular_localization, value= Nucleus.
Key=sequence_similarities, value=Belongs to the E2F/DP family.
Key=tissue_specificity, value= Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Key=protein_function, value= eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 133133|sc 133209|sc 136972|sc 166536|sc 28851|sc 31886|sc 31888|sc 9978
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of human EIF2S2, identical to the related mouse and rat sequences.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=38 kDa
Key=custom_attribute_2, value=WB: human HepG2 whole cell, human 293T whole cell, human Hela whole cell
Key=custom_attribute_3, value=BA3422
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Anti-EIF2S2 Antibody Picoband®

EIF2 beta antibody

Boster Bio Anti-EIF2S2 Antibody catalog # PA2157. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA2157
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-EIF2S2 Antibody Picoband®

View all EIF2 beta Antibodies

SKU/Catalog Number

PA2157

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EIF2S2 Antibody catalog # PA2157. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EIF2S2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2157)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human EIF2S2, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2157 is reactive to EIF2S2 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

38 kDa

Calculated molecular weight

38388 MW

Background of EIF2 beta

Eukaryotic translation initiation factor 2 subunit 2, also called EIF2S2 is a protein that in humans is encoded by the EIF2S2 gene. This gene belongs to the EIF-2-beta family. EIF2S2 is mapped to 20q11.2. Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA2157 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: human HepG2 whole cell, human 293T whole cell, human Hela whole cell

Validation Images & Assay Conditions

Gene/Protein Information For EIF2S2 (Source: Uniprot.org, NCBI)

Gene Name

EIF2S2

Full Name

Eukaryotic translation initiation factor 2 subunit 2

Weight

38388 MW

Superfamily

eIF-2-beta/eIF-5 family

Alternative Names

Eukaryotic translation initiation factor 2 subunit 2;Eukaryotic translation initiation factor 2 subunit beta;eIF-2-beta;EIF2S2;EIF2B; EIF2S2 EIF2, EIF2B, EIF2beta, PPP1R67, eIF-2-beta eukaryotic translation initiation factor 2 subunit beta eukaryotic translation initiation factor 2 subunit 2|eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa|protein phosphatase 1, regulatory subunit 67

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EIF2S2, check out the EIF2S2 Infographic

EIF2S2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EIF2S2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-EIF2S2 Antibody Picoband®

Question

We are currently using anti-EIF2S2 antibody PA2157 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-25

Answer

The anti-EIF2S2 antibody (PA2157) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-25

Order DetailsPrice
PA2157

100μg

$370
PA2157-10ug

10μg sample (liquid)

$99
PA2157-Biotin

100 μg Biotin conjugated

$570
PA2157-Cy3

100 μg Cy3 conjugated

$570
PA2157-Dylight488

100 μg Dylight488 conjugated

$570
PA2157-Dylight550

100 μg Dylight550 conjugated

$570
PA2157-Dylight594

100 μg Dylight594 conjugated

$570
PA2157-FITC

100 μg FITC conjugated

$570
PA2157-HRP

100 μg HRP conjugated

$570
PA2157-APC

100 μg APC conjugated

$670
PA2157-PE

100 μg PE conjugated

$670
PA2157-iFluor647

100 μg iFluor647 conjugated

$670
PA2157-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA2157
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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