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Product Info Summary
| SKU: | MA1054 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Pig |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-Laminin LAMA1 Monoclonal Antibody
SKU/Catalog Number
MA1054
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Laminin LAMA1 Monoclonal Antibody catalog # MA1054. Tested in IHC applications. This antibody reacts with Human, Pig.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Laminin LAMA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1054)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
LAM-89
Isotype
Mouse IgG1
Immunogen
Human laminin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1054 is reactive to LAMA1 in Human, Pig
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
68 kDa
Calculated molecular weight
196474 MW
Background of Lama1
Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1,beta-1 and gamma-1, formerly called beta-2 (LAMA2). This gene is over 260,000 base pairs and contains 64 exons. Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins. And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1054 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, feline, pig, By Heat
Positive Control
IHC: human placenta tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Laminin using anti-Laminin antibody (MA1054).
Laminin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Laminin Antibody (MA1054) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For LAMA1 (Source: Uniprot.org, NCBI)
Gene Name
LAMA1
Full Name
Laminin subunit alpha-1
Weight
196474 MW
Alternative Names
Laminin subunit beta-2;Laminin chain B3;Laminin-11 subunit beta;Laminin-14 subunit beta;Laminin-15 subunit beta;Laminin-3 subunit beta;Laminin-4 subunit beta;Laminin-7 subunit beta;Laminin-9 subunit beta;S-laminin subunit beta;S-LAM beta;Lamb2; Lama1|AA408497, L, Lama|laminin, alpha 1|laminin subunit alpha-1|S-LAM alpha|S-laminin subunit alpha|laminin A chain|laminin-1 subunit alpha|laminin-3 subunit alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LAMA1, check out the LAMA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LAMA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Laminin LAMA1 Monoclonal Antibody (MA1054)
Hello CJ!
MA1054 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang J,Chang H,Yin R,Xu S,Wang H,Gao Y. A temporal study on musculoskeletal morphology and metabolism in hibernating Daurian ground squirrels (Spermophilus dauricus). Bone. 2020 Dec 18:115826.doi: 10.1016/j.bone.2020.115826.Epub ahead of print.PMID: 3334
Species: Daurian ground squirrels
MA1054 usage in article: APP:IHC, SAMPLE:MUSCLE TISSUE, DILUTION:1:500
Kong XT, Wang Z, Mou JJ, Li CS, Xue HL, Wu M, Chen L, Xu JH, Xu LX. Change on apoptosis, autophagy and mitochondria of the Harderian gland in Cricetulus barabensis during age. Comp Biochem Physiol B Biochem Mol Biol. 2020 Dec 16:110547. doi: 10.1016/j.cbp
Species: Hamster
MA1054 usage in article: APP:IHC, SAMPLE:HG TISSUE, DILUTION:1:500
Li T, Yang Z, Cai Hj, Song Lw, Lu Ky, Zhou Z, Wu Zd. World J Gastroenterol. 2010 Feb 14;16(6):749-54. Effects Of In Vitro Cultivated Calculus Bovis Compound On Pulmonary Lesions In Rabbits With Schistosomiasis.
Zhu B, Xu T, Yuan J, Guo X, Liu D. Plos One. 2013 Sep 19;8(9):E76282. Doi: 10.1371/Journal.Pone.0076282. Ecollection 2013. Transcriptome Sequencing Reveals Differences Between Primary And Secondary Hair Follicle-Derived Dermal Papilla Cells Of The...
Chen W, Mo W, Sun K, Huang X, Zhang Yl, Song Hy. Curr Eye Res. 2009 Dec;34(12):1057-64. Doi: 10.3109/02713680903308487. Microplasmin Degrades Fibronectin And Laminin At Vitreoretinal Interface And Outer Retina During Enzymatic Vitrectomy.
Chen Tl, Wang Hl, Liu Yh, Fang Y, Tan Rz, Zhou Ph, Zhou Q, Lv Xy. Scientificworldjournal. 2014;2014:682189. Doi: 10.1155/2014/682189. Epub 2014 Jul 14. Highly Effective Ex Vivo Gene Manipulation To Study Kidney Development Using Self-Complementary...
Cheng Y, Wang Y, Kang Yz, Hu Py, Gao Y, Pan Mx. Digestion. 2013;87(3):189-95. Doi: 10.1159/000349949. Epub 2013 May 1. In Vitro Culture Of Tumour-Derived Hepatocytes In Decellularised Whole-Liver Biological Scaffolds.
He S, Xia T, Wang H, Wei L, Luo X, Li X. Acta Biomater. 2012 Jul;8(7):2659-69. Doi: 10.1016/J.Actbio.2012.03.044. Epub 2012 Apr 3. Multiple Release Of Polyplexes Of Plasmids Vegf And Bfgf From Electrospun Fibrous Scaffolds Towards Regeneration Of ...
Liu Y, Lu J, Li H, Wei J, Li X. Acta Biomater. 2015 Jan;11:114-25. Doi: 10.1016/J.Actbio.2014.10.004. Epub 2014 Oct 7. Engineering Blood Vessels Through Micropatterned Co-Culture Of Vascular Endothelial And Smooth Muscle Cells On Bilayered Electro...
Chen F, Wan H, Xia T, Guo X, Wang H, Liu Y, Li X. Eur J Pharm Biopharm. 2013 Nov;85(3 Pt A):699-710. Doi: 10.1016/J.Ejpb.2013.07.009. Epub 2013 Jul 24. Promoted Regeneration Of Mature Blood Vessels By Electrospun Fibers With Loaded Multiple Pdna-C...
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Customer Q&As
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2 Customer Q&As for Anti-Laminin LAMA1 Monoclonal Antibody
Question
Does MA1054 react with rat samples?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
MA1054 does not react with rat samples.
Boster Scientific Support
Answered: 2020-02-18
Question
Is this monoclonal antibody still available from you. if yes can you let me know if this has cross reactivity to rat and if it will work for IHC-paraffin section on rat tissue
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
MA1054 doedn't react with rat samples. Our lab hasn't got any
Boster Scientific Support
Answered: 2017-10-06
