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Product Info Summary
| SKU: | EK1287 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse soluble CD6 ELISA Kit PicoKine®
SKU/Catalog Number
EK1287
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse soluble CD6 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse soluble CD6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1287)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L18-G396
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1287 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1287 is reactive to Cd6 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1287 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD6
CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What’s more, this gene may be associated with susceptibility to multiple sclerosis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1287-CAP | Anti-Mouse Cd6 Pre-coated 96-well strip microplate | 1 |
| EK1287-ST | Mouse Cd6 Standard | 2 vials, 10 ng/tube |
| EK1287-DA | Mouse Cd6 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 | |
| O.D. | 0.014 | 0.059 | 0.1 | 0.185 | 0.349 | 0.666 | 1.217 | 2.030 |
Data Example Images
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Mouse CD6 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD6, Dilution ratio of 1:1, concentration in serum and plasma is 90-248 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse soluble CD6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 133 | 525 | 1268 | 129 | 485 | 1288 |
| Standard deviation | 5.58 | 38.9%7 | 92.56 | 6.45 | 37.83 | 114.63 |
| CV (%) | 4.2% | 6.7% | 7.3% | 5% | 7.8% | 8.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD6 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 133 | 133 | 120 | 119 | 126 | 6.75 | 5.3% |
| Sample 2 | 525 | 507 | 540 | 469 | 510 | 26.52 | 5.2% |
| Sample 3 | 1268 | 1267 | 1220 | 1339 | 1273 | 42.5 | 3.3% |
Protein Target Info & Infographic
Gene/Protein Information For Cd6 (Source: Uniprot.Org, NCBI)
Gene Name
Cd6
Full Name
T-cell differentiation antigen CD6
Weight
72255 MW
Alternative Names
T-cell differentiation antigen CD6;CD6;Cd6; CD6 TP120 CD6 molecule T-cell differentiation CD6|CD6 |T12
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cd6, check out the Cd6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse soluble CD6 ELISA Kit PicoKine® (EK1287)
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Customer Q&As
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6 Customer Q&As for Mouse soluble CD6 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-12-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-12-23
Question
Q: What is the optimal O.D. value for CD6 ELISA kit? I performed your CD6 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD6 even though the O.D. values are not very high?
M. Puri
Verified customer
Asked: 2020-02-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD6 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD6 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-02-18
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CD6 in Mouse cell lysates? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
M. Robinson
Verified customer
Asked: 2018-04-04
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD6 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-04-04
Question
Q: which procedure should I follow in order to thaw whole blood sample for CD6 ELISA after freezing?
W. Edwards
Verified customer
Asked: 2018-02-14
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD6 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-02-14
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
H. Baker
Verified customer
Asked: 2018-01-11
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-01-11
Question
Q: is it okay to use citrate plasma as samples in Mouse CD6 Picokine® ELISA Kit (Catalog # EK1287)?
Verified Customer
Verified customer
Asked: 2016-09-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CD6 causing degradation of its protein structure. CD6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CD6 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-09-23
