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Product Info Summary
| SKU: | FEK1414 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Mouse CD5L/CT-2 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1414
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CD5L / CT 2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Cd5l in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Mouse CD5L/CT-2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1414)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E22-V352
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1414 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1414 is reactive to Cd5l in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK1414 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD5L
CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1414-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1414-ST | Mouse CD5L Standard | 2 | 10 ng/tube |
| FEK1414-A | Mouse Cd5l Antibody A | 1 | 3ml |
| FEK1414-B | Mouse Cd5l Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.004 | 0.131 | 0.209 | 0.424 | 0.747 | 1.294 | 1.996 | 2.436 |
Data Example Images
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Mouse CD5L PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CD5L/CT-2 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 201 | 1789 | 4940 | 207 | 1935 | 4821 |
| Standard deviation | 13.26 | 110.91 | 375.44 | 17.38 | 131.58 | 414.6 |
| CV (%) | 6.6% | 6.2% | 7.6% | 8.4% | 6.8% | 8.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD5L in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 201 | 236 | 232 | 222 | 222 | 13.55 | 6.1% |
| Sample 2 | 1789 | 1795 | 1875 | 1980 | 1859 | 77.28 | 4.1% |
| Sample 3 | 4940 | 4445 | 4825 | 4834 | 4761 | 187.96 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For Cd5l (Source: Uniprot.Org, NCBI)
Gene Name
Cd5l
Full Name
CD5 antigen-like
Weight
38863 MW
Alternative Names
CD5 antigen-like;Apoptosis inhibitor expressed by macrophages ;mAIM ;Apoptosis inhibitory 6;SP-alpha ;Cd5l;Aim , Api6; CD5L AIM, API6, CT-2, PRO229, SP-ALPHA, Spalpha, hAIM CD5 molecule like CD5 -like|CD5 -like (scavenger receptor cysteine rich family)|apoptosis inhibitor 6|apoptosis inhibitor expressed by macrophages|apoptosis inhibitor of macrophage|igM-associated peptide
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cd5l, check out the Cd5l Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd5l: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CD5L/CT-2 PicoKine® Quick ELISA Kit (FEK1414)
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