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Product Info Summary
| SKU: | FEK1315 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human CD21/CR2 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1315
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD21/CR2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human CD21/CR2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1315)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Ile21-71
Sensitivity
<20 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1315 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1315 is reactive to CR2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK1315 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CR2
Complement receptor type 2 (CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9).
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1315-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1315-ST | Human CR2 Standard | 2 | 50 ng/tube |
| FEK1315-A | Human CR2 Antibody A | 1 | 3ml |
| FEK1315-B | Human CR2 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.014 | 0.096 | 0.168 | 0.317 | 0.565 | 0.938 | 1.757 | 2.382 |
Data Example Images
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Human CD21 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD21/CR2 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 59 | 260 | 913 | 58 | 268 | 861 |
| Standard deviation | 3.36 | 12.22 | 49.30 | 4.40 | 13.93 | 55.96 |
| CV(%) | 5.7% | 4.7% | 5.4% | 7.6% | 5.2% | 6.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CR2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1242 | 1468 | 1273 | 1362 | 1336 | 87.89 | 6.5% |
| Sample 2 | 7916 | 7621 | 6548 | 6832 | 7229 | 558.34 | 7.7% |
| Sample 3 | 24012 | 23247 | 26467 | 22418 | 24036 | 1512.51 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For CR2 (Source: Uniprot.Org, NCBI)
Gene Name
CR2
Full Name
Complement receptor type 2
Weight
112916 MW
Superfamily
receptors of complement activation (RCA) family
Alternative Names
Complement receptor type 2;Cr2;Complement C3d receptor;Epstein-Barr virus receptor;EBV receptor;CD21;CR2;C3DR; CR2 C3DR, CD21, CR, CVID7, SLEB9 complement C3d receptor 2 complement receptor type 2|EBV receptor|complement component (3d/Epstein Barr virus) receptor 2|complement component 3d receptor 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CR2, check out the CR2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD21/CR2 PicoKine® Quick ELISA Kit (FEK1315)
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Customer Q&As
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1 Customer Q&As for Human CD21/CR2 PicoKine® Quick ELISA Kit
Question
Is the epitope of Human CD21/CR2 PicoKine intracellular?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Indeed, the epitope of this Human CD21/CR2 PicoKine antibody is intracellular, thus it is required to permeabilize the cells for flow cytometry.
Boster Scientific Support
Answered: 2019-05-31
