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Product Info Summary
| SKU: | M03913-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, WB |
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Product info
Product Name
Anti-Hu CD6 Purified Monoclonal Antibody
SKU/Catalog Number
M03913-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD6 Purified Monoclonal Antibody (Catalog# M03913-2). Tested in Flow Cytometry, IP, WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD6 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03913-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-98
Isotype
Mouse IgG1
Immunogen
Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone). The antibody MEM-98 reacts with an extracellular epitope of CD6, a 100-130 kDa single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M03913-2 is reactive to CD6 in Human
Reconstitution
Observed Molecular Weight
100 kDa
Calculated molecular weight
71801 MW
Background of CD6
CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03913-2 is guaranteed for Flow Cytometry, IP, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: Recommended dilution: 2 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen.
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.
Validation Images & Assay Conditions
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Anti-Hu CD6 Purified (M03913-2) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Molt-4 and HeLa cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody M03913-2 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed. CD6 molecules were detected at ~100 kDa in Molt-4 cell line under non-reducing conditions.
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD6 (M03913-2) purified antibody (concentration in sample 2 μg/ml, GAM APC).
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Separation of human CD6 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD6 (M03913-2) purified antibody (concentration in sample 2 μg/ml, GAM APC).
Protein Target Info & Infographic
Gene/Protein Information For CD6 (Source: Uniprot.org, NCBI)
Gene Name
CD6
Full Name
T-cell differentiation antigen CD6
Weight
71801 MW
Alternative Names
T12, TP120 CD6 TP120 CD6 molecule T-cell differentiation CD6|CD6 |T12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD6, check out the CD6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD6 Purified Monoclonal Antibody (M03913-2)
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