Human CD5L/CT-2 PicoKine® Quick ELISA Kit

Product Name

Human CD5L/CT-2 PicoKine® Quick ELISA Kit

See all CD5L products

SKU/Catalog Number

FEK1413

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD5L / CT 2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD5L in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human CD5L/CT-2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1413)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: S20-G347

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK1413 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1413 is reactive to CD5L in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK1413 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CD5L

CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.

Kit Components

Catalog number	Description	Quantity	Volume
FEK1413-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK1413-ST	Human CD5L Standard	2	10 ng/tube
FEK1413-A	Human CD5L Antibody A	1	3ml
FEK1413-B	Human CD5L Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.003	0.468	0.870	1.303	2.183	2.525	2.536	2.524

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD5L/CT-2 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD5L in ELISA kits from four different production batches/lots.

Gene/Protein Information For CD5L (Source: Uniprot.Org, NCBI)

Alternative Names

CD5 antigen-like;Apoptosis inhibitor expressed by macrophages ;hAIM ;CT-2 ;IgM-associated peptide ;SP-alpha ;CD5L;API6;UNQ203/PRO229 ; CD5L AIM, API6, CT-2, PRO229, SP-ALPHA, Spalpha, hAIM CD5 molecule like CD5 -like|CD5 -like (scavenger receptor cysteine rich family)|apoptosis inhibitor 6|apoptosis inhibitor expressed by macrophages|apoptosis inhibitor of macrophage|igM-associated peptide

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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