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From Overwhelmed To Overachieving
ELISA SERVICE
We will run your plates and you will get more done. Boster Bio offers easy-to-work-with ELISA services at fast turnaround time.
Get a quote today if you want your ELISA...
- run by one of the largest ELISA manufacturing company
- to yield robust and reproducible data
- turnaround in a fast and professional manner
-Multiplex ELISA available
-Custom ELISA development available
Begin Inquiry
Why Choose Boster For ELISA CRO Service?
Because we are an antibody manufacturer with ELISA expertise
With over 30 years of experience, Boster excels in providing comprehensive support for IND filing, GMP release assays, and various advanced requirements for therapeutic and diagnostic clients. Since its establishment in 1993, Boster has been a leading manufacturer of antibodies and ELISA kits, catering to scientists worldwide. Our founder pioneered the development of Picokine™, an exclusive sandwich ELISA platform known for its exceptional sensitivity, in the early 2000s. Throughout the last two decades, we have successfully created over 1,000 highly sensitive Picokine™ ELISA kits, which have been extensively referenced in more than 6,000 publications up until 2019, as verified by reputable sources such as Google Scholar, Bioz, and CiteAb. At Boster Bio, our team of ELISA experts diligently performs hundreds of ELISA assays every week. Leveraging our extensive history and large-scale operations, Boster Bio offers a distinct blend of service quality, specialized knowledge, and cost-effectiveness, making us the ideal choice for streamlining your workflow and fulfilling your ELISA service needs.
Their (Boster Bio) service exceeded my expectations. Lana responds quickly (within 1-2 hours) with professionalism. Good quality of data using low amount of sample volume at a fair price.
--Research Scientist from University of Texas at Austin
Getting started
Understand ELISA options in the SOW to get a quote ASAP
Overview
Quote, SOW, and sample manifest
In order to provide a quote for your ELISA project, we require a clear definition of your scope of work (SOW). The following section presents an example of an ELISA SOW, outlining various options in detail. If you are interested in commencing your project with us, we kindly request that you carefully review this section and provide the essential details in your inquiry.
Once we have received all the necessary information from you and finalized your SOW, we will prepare a quote for your consideration. Upon placing an order, please ensure to include a sample manifest along with the order. You can access the sample manifest template through the link provided below:
Fill out the scope of work
When we have all necessary information, we will issue you a quote. Contact us at [email protected] for any questions.
SOW template for ELISA service
Project Scope:
We will perform ELISA for the client.
Options:
[Sample dilution optimization: yes / no (customer needs to provide sample dilution ratio)]
Excess materials: [return to customer/destroy]
1. Materials
Sample information and experiment layout:
Sample 1: (example entry) 80 human sera, to test in duplicates. Samples need no processing. This sample is a regular sample.
Sample 2: [Sample Qty] [Species] [Sample type], test in [Sample replication]. Samples need [Sample processing requirements]. This sample is [Sample role: 1. to be used in optimization 2. To be used as positive/negative control 3. a regular sample]
ELISA kits and materials:
ELISA kit 1: (example entry) Boster BDNF EK0307 $399, Source: Boster
ELISA kit 2: [Company] [Target] [Sku] $[Price], [Source: customer provide/procure/Boster]. [Optional: is a DIY kit: yes / no]
2. Experiment Phases
Phase 1: sample concentrations optimization
We will use up to 5 samples to optimize the dilution for the samples for each kit. We will test 8 concentrations of each sample to decide the best dilution to use in the next phase.
Note: if the customer knows the dilution ratio(s) to use, we will take the suggestion and skip this phase. If we find out in this phase that samples have large variations in target protein levels, in the next phase multiple dilutions should be run for each sample to ensure each sample has data in the linear range of the assay.
Deliverable: a report of the optimization results.
Turnaround time: 1-2 weeks after all materials are received.
Phase 2: ELISA of all samples
We will perform ELISA for all samples using all ELISA kits.
Deliverable: a report of the final results
Turnaround time: 1-2 weeks after customer confirmation of phase 1 and all materials are received
Special requirements: no
SOW options explained
Sample dilution optimization: we will determine the ideal dilution factor for your samples. Selecting up to 5 samples, we will perform a 7-fold serial dilution to assess which dilution ratio can bring the analyte's concentration to within the assay's dynamic range, preventing saturation or detection issues. Sample dilution optimization is necessary unless you can provide the exact dilution ratio to use for the samples.
Return or despose excess materials: The remaining samples and reagents be shipped back to you or disposed of. There are fees associated with both options
Sample type and processing requirements: different sample types require different processing. Details see below.
| Protein Extraction | Clarification* | BCA | |
|---|---|---|---|
| Cells | Yes | Yes | Yes |
| Lysate | No | Yes | Yes |
| Bone Tissue | Yes (extra fee) | Yes | Yes |
| Tissue | Yes | Yes | Yes |
| Cell Culture Supernatant | No | No | Yes |
| Cell Pellet | Yes | Yes | Yes |
| Plasma** | No | No | No |
| Serum** | No | No | No |
| Blood*** | No | No | No |
| PBMC's | No | No | No |
| Urin**** | Afyon | No | Yes |
| Milk**** | Afyon | No | Yes |
| Saliva**** | Afyon | No | Yes |
*Clarification: filtering the sample to get rid of cellular debris. Helps with reducing ELISA background.
**Serum and Plasma: 1-1.5uL of sample per lane, protein concentration assumed to be ~50mg/mL, need clarification if samples are lipemic
***Blood: need to spin down to serum/plasma, extra charge
****Low expression protein: Need to use Afyon kit to concentrate protein. Extra charge.
Sample replication: by default we assume all samples are tested in duplicates. If you want it done differently please tell us.
Develop it yourself (DIY) kit: if this kit is not a pre-coated, ready to use ELISA kit, but an
antibody pair + recombinant standard, we will have to develop the
assay, and charge a fee for it.
Sample role in experiment: the 3 most common sample roles in
ELISA are:
1. used in optimization
2. used as positive or
negative control
3. a regular sample, aka a Unknown
Special requirements: anything not covered in the SOW template and the sample manifest can be written here.
Unlock the power of assay services today! Our comprehensive range of assay services will empower you to uncover critical insights and make significant breakthroughs in your research.
What's next?
Submit inquiry, get quote, place order, send materials, get data
Once you have submitted your inquiry, we will try to match your information to our SOW template, and issue you a quote if we have enough information.
Once you receive the quote, you can place the order with us by emailing [email protected]. Be sure to include the sample manifest with your order.
Before sending us your materials, please carefully read Sample Collection And Submission Guidelines. After receiving all necessary materials, we will start your project as soon as we can. The typical turnaround time is 1-2 weeks each phase. You will get your professional ELISA data report back and can schedule a meeting with us to discuss further.
Sample Report
Get a Quote