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Product Info Summary
| SKU: | PB10051 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD40 Antibody Picoband®
View all CD40/TNFRSF5 Antibodies
SKU/Catalog Number
PB10051
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD40 Antibody Picoband® catalog # PB10051. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD40 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10051)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD40/TNFRSF5 recombinant protein (Position: E21-R193). Human CD40/TNFRSF5 shares 60.2% amino acid (aa) sequence identity with mouse CD40/TNFRSF5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10051 is reactive to CD40 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
30619 MW
Background of CD40/TNFRSF5
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. It is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10051 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Raji whole cell
IHC: human tonsil tissue
FCM: human PBMC cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10051).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40/TNFRSF5 antigen affinity purified polyclonal antibody (Catalog # PB10051) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40/TNFRSF5 at approximately 43 kDa. The expected band size for CD40/TNFRSF5 is at 30 kDa.
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Figure 2. IHC analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10051).
CD40/TNFRSF5 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD40/TNFRSF5 Antibody (PB10051) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. Flow Cytometry analysis of human PBMC cells using anti-CD40/TNFRSF5 antibody (PB10051).
Overlay histogram showing human PBMC cells stained with PB10051 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD40/TNFRSF5 Antibody (PB10051, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CD40 (Source: Uniprot.org, NCBI)
Gene Name
CD40
Full Name
Tumor necrosis factor receptor superfamily member 5
Weight
30619 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CDw40;CD40;CD40;TNFRSF5; CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD40, check out the CD40 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD40 Antibody Picoband® (PB10051)
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Customer Q&As
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15 Customer Q&As for Anti-CD40 Antibody Picoband®
Question
I was wanting to use to test anti-CD40 antibody PB10051 on human kidney for research purposes, then I may be interested in using anti-CD40 antibody PB10051 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The products we sell, including anti-CD40 antibody PB10051, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-06
Question
Would anti-CD40 antibody PB10051 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
According to the expression profile of kidney, CD40 is highly expressed in kidney. So, it is likely that anti-CD40 antibody PB10051 will work for WB with kidney.
Boster Scientific Support
Answered: 2020-02-28
Question
We are currently using anti-CD40 antibody PB10051 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The anti-CD40 antibody (PB10051) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-02
Question
See below the WB image, lot number and protocol we used for kidney using anti-CD40 antibody PB10051. Please let me know if you require anything else.
G. Huang
Verified customer
Asked: 2019-12-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-CD40 antibody PB10051. Let me know if you need anything else.
K. Walker
Verified customer
Asked: 2019-06-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-26
Question
My question regarding product PB10051, anti-CD40 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10051 anti-CD40 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-19
Question
My team were well pleased with the WB result of your anti-CD40 antibody. However we have seen positive staining in kidney isoform i: cell membrane using this antibody. Is that expected? Could you tell me where is CD40 supposed to be expressed?
J. Anderson
Verified customer
Asked: 2018-07-24
Answer
From literature, kidney does express CD40. Generally CD40 expresses in isoform i: cell membrane. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161
Boster Scientific Support
Answered: 2018-07-24
Question
I was wanting to use your anti-CD40 antibody for WB for human kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human kidney identification?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
You can see on the product datasheet, PB10051 anti-CD40 antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-19
Question
Our lab used your anti-CD40 antibody for WB on left coronary artery in a previous project. I am using human, and We are going to use the antibody for ELISA next. We are interested in examining left coronary artery as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
D. Li
Verified customer
Asked: 2017-11-29
Answer
I looked at the website and datasheets of our anti-CD40 antibody and it seems that PB10051 has been validated on human in both WB and ELISA. Thus PB10051 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-11-29
Question
Is a blocking peptide available for product anti-CD40 antibody (PB10051)?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
We do provide the blocking peptide for product anti-CD40 antibody (PB10051). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-15
Question
Will PB10051 anti-CD40 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-29
Answer
It shows on the product datasheet, PB10051 anti-CD40 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-29
Question
Is this PB10051 anti-CD40 antibody reactive to the isotypes of CD40?
M. Evans
Verified customer
Asked: 2017-02-08
Answer
The immunogen of PB10051 anti-CD40 antibody is E. coli-derived human CD40/TNFRSF5 recombinant protein (Position: E21-R193). Human CD40/TNFRSF5 shares 60.2% amino acid (aa) sequence identity with mouse CD40/TNFRSF5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-02-08
Question
We have been able to see staining in human ovary. Do you have any suggestions? Is anti-CD40 antibody supposed to stain ovary positively?
D. Jones
Verified customer
Asked: 2017-02-01
Answer
From literature ovary does express CD40. From Uniprot.org, CD40 is expressed in left coronary artery, kidney, ovary, leukocyte, lymphoma urinary bladder carcinoma, among other tissues. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161
Boster Scientific Support
Answered: 2017-02-01
Question
Do you have a BSA free version of anti-CD40 antibody PB10051 available?
M. Walker
Verified customer
Asked: 2016-03-15
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD40 antibody PB10051 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-03-15
Question
I see that the anti-CD40 antibody PB10051 works with WB, what is the protocol used to produce the result images on the product page?
E. Williams
Verified customer
Asked: 2014-06-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-06-05
