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Product Info Summary
| SKU: | PB9911 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ZBTB7A Antibody Picoband®
View all ZBTB7A/Pokemon Antibodies
SKU/Catalog Number
PB9911
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZBTB7A Antibody Picoband® catalog # PB9911. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZBTB7A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9911)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse ZBTB7A, different from the related human sequence by eleven amino acids, and from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9911 is reactive to Zbtb7a in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
60281 MW
Background of ZBTB7A/Pokemon
Zinc finger and BTB domain-containing protein 7A is a protein that in humans is encoded by the ZBTB7A gene. ZBTB7A has a critical oncosuppressive role in the prostate. Prostate-specific inactivation of ZBTB7A leads to a marked acceleration of PTEN loss-driven prostate tumorigenesis through bypass of PTEN loss-induced cellular senescence. It has been showed that ZBTB7A physically interacts with SOX9 and functionally antagonizes its transcriptional activity on key target genes such as MIA, which is involved in tumor cell invasion, and H19, a long noncoding RNA precursor for an RB-targeting microRNA. Inactivation of ZBTB7A in vivo leads to RB downregulation, bypass of PTEN loss-induced cellular senescence, and invasive prostate cancer. Notably, it has been also found that ZBTB7A is genetically lost, as well as downregulated at both the mRNA and protein levels, in a subset of human advanced prostate cancers. Therefore, ZBTB7A is identified as a context-dependent cancer gene that can act as an oncogene in some contexts but that also has oncosuppressive-like activity in PTEN-null tumors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9911 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse kidney tissue, NIH/3T3 whole cell
IHC: mouse intestine tissue, rat brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ZBTB7A using anti-ZBTB7A antibody (PB9911).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse kidney tissue lysates,
Lane 2: NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB7A antigen affinity purified polyclonal antibody (Catalog # PB9911) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB7A at approximately 75 kDa. The expected band size for ZBTB7A is at 60 kDa.
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Figure 2. IHC analysis of ZBTB7A using anti-ZBTB7A antibody (PB9911).
ZBTB7A was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZBTB7A Antibody (PB9911) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of ZBTB7A using anti-ZBTB7A antibody (PB9911).
ZBTB7A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZBTB7A Antibody (PB9911) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Zbtb7a (Source: Uniprot.org, NCBI)
Gene Name
Zbtb7a
Full Name
Zinc finger and BTB domain-containing protein 7A
Weight
60281 MW
Alternative Names
Zinc finger and BTB domain-containing protein 7A;Leukemia/lymphoma-related factor;POZ and Krueppel erythroid myeloid ontogenic factor;POK erythroid myeloid ontogenic factor;Pokemon;Zbtb7a;Lrf, Zbtb7; Zbtb7a|9030619K07Rik, 9130006G12Rik, AI452336, FBI-, FBI-1, L, Lrf, Pok, Pokemon, Zbtb7|zinc finger and BTB domain containing 7a|zinc finger and BTB domain-containing protein 7A|POK erythroid myeloid ontogenic factor|POZ and Krueppel erythroid myeloid ontogenic factor|leukemia/lymphoma related factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Zbtb7a, check out the Zbtb7a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Zbtb7a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZBTB7A Antibody Picoband® (PB9911)
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Customer Q&As
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1 Customer Q&As for Anti-ZBTB7A Antibody Picoband®
Question
We are currently using anti-ZBTB7A antibody PB9911 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on horse tissues as well?
S. Bhatt
Verified customer
Asked: 2017-03-07
Answer
The anti-ZBTB7A antibody (PB9911) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-07
