Anti-RECK Antibody Picoband®

Product Name

Anti-RECK Antibody Picoband®

View all RECK Antibodies

SKU/Catalog Number

A06439-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RECK Antibody Picoband® catalog # A06439-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RECK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06439-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human RECK, which shares 88.6% and 79.5% amino acid (aa) sequence identity with mouse and rat RECK, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06439-1 is reactive to RECK in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

110 kDa

Calculated molecular weight

106.457kDa

Background of RECK

Reversion-inducing-cysteine-rich protein with kazal motifs, also known as RECK, is a human gene, thought to be a metastasis suppressor. The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A06439-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Positive Control

WB: human MOLT-4 whole cell, human U87 whole cell

Validation Images & Assay Conditions

Gene/Protein Information For RECK (Source: Uniprot.org, NCBI)

Alternative Names

Reversion-inducing cysteine-rich protein with Kazal motifs; hRECK; Suppressor of tumorigenicity 15 protein; RECK; ST15 RECK ST15 reversion inducing cysteine rich protein with kazal motifs reversion-inducing cysteine-rich protein with Kazal motifs|membrane-anchored glycoprotein (metastasis and invasion)|suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein with kazal motifs)|suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein with kazal motifs)|suppressor of tumorigenicity 15 protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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