Zbtb7a Antibodies

Zinc finger and BTB domain-containing protein 7A (Zbtb7a)

Plays a key role in the education of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. Specifically represses the transcription of the CDKN2A gene.

Efficiently abrogates E2F1- dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'.

Gene Information

Mouse
Gene Name:	Zbtb7a
Uniprot:	O88939
Entrez:	16969

Family

Belongs to:
No superfamily

Alternative Names

DKFZp547O146; Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; FBI-1; FBI-1POK erythroid myeloid ontogenic factor; FBI1TTF-I-interacting peptide 21; HIV-1 inducer of short transcripts binding protein; Leukemia/lymphoma-related factor; LRF; LRFHIV-1 1st-binding protein 1; lymphoma related factor; MGC99631; Pokemon; TIP21; ZBTB7A; ZBTB7zinc finger and BTB domain containing 7; zinc finger and BTB domain containing 7A; zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcriptsbinding protein; zinc finger and BTB domain-containing protein

Molecular Weight

Mass (kDA):
60.281 kDA

Genomic Context

Mouse
Location:	10|10 C1
Sequence:	10;

Tissue Specificity

Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In the spleen, mainly expressed in the white pulp germinal centers (at protein level). Up-regulated in thymic lymphomas.

Diseases

• Malignant Neoplasms
• Cell Transformation, Neoplastic
• Neoplasms
• Carcinoma
• Lymphoma
• Liver Carcinoma
• Malignant Paraganglionic Neoplasm
• Malignant Neoplasm Of Lung
• Malignant Neoplasm Of Breast
• Liver Neoplasms
• Lung Neoplasms
• Neoplasm Metastasis
• Immunologic Deficiency Syndromes

• Mammary Neoplasms
• Non-small Cell Lung Carcinoma
• Prostatic Neoplasms
• Metaplastic Cell Transformation
• Carcinogenesis
• Malignant Neoplasm Of Prostate
• B-cell Lymphomas

Pathways

• Oncogenesis
• Cell Proliferation
• Cell Cycle
• Cell Differentiation
• Cell Cycle Arrest
• Localization
• Senescence
• Pathogenesis
• Rna Interference
• Osteoclast Differentiation
• Cell Migration
• Histone Deacetylation
• Cell Death

• Cell Growth
• Osteoclast Development
• Programmed Cell Death
• Cellular Homeostasis
• Cell Fate Determination
• Myelination
• Cell Maturation

PTMs

• Deacetylation

• Sumoylation

• Phosphorothioation

Research Areas

• Cancer
• Transcription Factors and Regulators

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Zbtb7a

Best Uses Of The ZBTB7A Marker

It is important to use high-affinity primary antibodies when using antibodies for Western Blot analysis. Boster antibodies have been validated on Immunohistochemistry, Western Blotting, and ELISA. Learn more about Boster's antibody and their best uses. Also, make sure to check out the high-affinity secondary antibodies and DAB chromogenic detect system.

Boster's high affinity primary antibodies

High-affinity prima antibodies use the ZBTBT7A markers to recognize proteins within fixed tissue. They have been proven to work in the research community. They are stable and have low variation from lot-to-lot. They are also highly specific, making high-throughput application possible. Here are some of these benefits of Boster’s high affinity primary antibodies using ZBTB7A.

Fluorescent microinjection can use three or more markers to detect a target. The primary antibodies that are fluorescent dye-conjugated to the markers are used to label them. These primary antibodies are often used in combination of fluorescent secondary antibodies to increase amplification. The available filter sets allow only three markers. Therefore, the process requires primary antibodies raised in different species and isotypes.

The ZBTB7A biomarker is highly specific. Different non-overlapping epitopes must be recognized by capture and detection antibodies. Cross-reactivity occurs when they recognize the same epitopes. This reduces sensitivity and dynamic range. Secondary antibodies are critical in indirect detection of biological agent. This means that you need two different antibodies to detect and capture biological agent.

Two antigens on the same tissue section can be visualized simultaneously or sequentially. You must choose the appropriate pigments for each antigen, and ensure that they don't block the visualization of the others. Light-emitting fluorophores are used to visualize two antigens. Secondary antibodies can not cross-react to primary antibodies in either of these directions.

Western blot antibody detection methods

There are many ways to detect Boster Bio ZBTB7A markers in Western blots. The secondary antibody bound to the substances will determine the method. Some methods use ECL chemiluminescent systems, while others use a chromogenic detector system. The Boster ECL Chemiluminescent method requires that the sample be exposed to secondary antibodies before being exposed to the appropriate substrate.

Depending on the method used, the primary antibody may be diluted with a buffer to reduce background. It is important to wash the membrane for only a few minutes as longer washing times reduce signal. The membrane is then incubated using a label antibody. The label antibody, usually horseradish peroxidase (or horseradish peroxidase), creates a signal that corresponds to the position of target protein on membrane. The signal is then captured on film and developed in dark rooms.

A negative control consists of a lysate taken from a protein not expressing the target protein. The antibody detects this band at 17KD. Secondary antibodies block the reaction to detect the band. A negative control is used to confirm that the antibody is not nonspecific. The negative control is a not-specific antibody. A positive control antibody detects the band at a smaller size.

Blocking is the next step in western blotting. Because they reduce background and non-specific binding of antibodies, blocking reagents is crucial. A typical blocking buffer consists 5% BSA or gelatin and TBST. These buffers can be found cheaply and are easily accessible. However, it is important to test them all to see which one gives the best signal. For the best results, use one of these standard blocking buffers.

You must wash the samples in the correct buffer. It is also important to properly prepare the sandwich. An inadequately prepared transfer sandwich may result in a uneven band. You should shake the membrane during incubation to avoid this. In addition, be sure to remove all the background before starting the transfer. In case you find an unfavorable band, the blocking agent may have bound to the target protein.

ECL chemiluminescent detection system

A luminol, a chemical, is used to detect protein on cell membranes. The reaction causes the emission of weak sunlight. To increase the intensity of the luminol's signal, enhancers can be added. The result is commonly known by the term enhanced chemiluminescence. Boster Bio's ECL Chemiluminescent detection system can detect a variety of wavelengths that are suitable for different applications.

Bovine anti goat IgG–HRP in PBST pH 7.4 at 22°C were the reagents used to detect protein band detection. Proteintech Group and Affinity Biosciences bought the protein-binding reagents. The chemiluminescent detection device can detect bands of at least 43 kDa.

The target enzyme dictates the substrate choice. Common substrates include Acridan-based reagents, luminol based HRP reagents, and 1,2-dioxetane based chemiluminescent substrata. The most widely used is the luminol HRP reagent. It contains 3-aminophthalate. This decomposes to a lower level of energy and releases photons.

An immunoassay must have a stable, long-lasting signal. Quantitative detection and high-sensitivity are crucial requirements. For strong signals, high-dilution factors are essential. Refer to the Kit Components List (available under Documents) for the best signal.

The Clarity Max Chemiluminescent Detection Systems are compatible to all horseradish Peroxidase Conjugates. These systems can be used for all Western Blot applications. Enhanced Chemiluminescent detection is based on the emission of light, and is most often used for protein detection in western blots. The secondary antibody used in western Blotting is conjugated to horseradish Peroxidase enzyme. This reaction results in light at 428 nm. The signal is then captured using a digital camera or X-ray film.

The optimal western blotting conditions can last from six to twenty-four hour. The enzyme-to substrate ratio and the substrate concentration will determine how long light generation takes place. Substrate concentration is relatively constant, while the amount of enzyme conjugate is largely variable. Excessive amounts of enzyme conjugate cause signal variability, dark background, and low sensitivity. Because each component is optimized, the ECL chemiluminescent detection device provides reproducible results.

DAB chromogenic detector system

DAB chromogenic detector system is a powerful tool to quickly quantify protein levels. The DAB enzyme causes a color shift from pink to blue when the protein is expressed in a cell. Boster Bio DAB systems can detect protein concentrations inside living cells. This system can detect the amount protein in a cell population by using a variety reagents including biotin-based, chromogenic staining.