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Product Info Summary
| SKU: | PB9910 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ZBTB7A Antibody Picoband®
View all ZBTB7A/Pokemon Antibodies
SKU/Catalog Number
PB9910
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZBTB7A Antibody Picoband® catalog # PB9910. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZBTB7A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9910)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ZBTB7A, different from the related mouse sequence by eleven amino acids, and from the related rat sequence by ten amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9910 is reactive to ZBTB7A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
61439 MW
Background of ZBTB7A/Pokemon
Zinc finger and BTB domain-containing protein 7A is a protein that in humans is encoded by the ZBTB7A gene. ZBTB7A has a critical oncosuppressive role in the prostate. Prostate-specific inactivation of ZBTB7A leads to a marked acceleration of PTEN loss-driven prostate tumorigenesis through bypass of PTEN loss-induced cellular senescence. It has been showed that ZBTB7A physically interacts with SOX9 and functionally antagonizes its transcriptional activity on key target genes such as MIA, which is involved in tumor cell invasion, and H19, a long noncoding RNA precursor for an RB-targeting microRNA. Inactivation of ZBTB7A in vivo leads to RB downregulation, bypass of PTEN loss-induced cellular senescence, and invasive prostate cancer. Notably, it has been also found that ZBTB7A is genetically lost, as well as downregulated at both the mRNA and protein levels, in a subset of human advanced prostate cancers. Therefore, ZBTB7A is identified as a context-dependent cancer gene that can act as an oncogene in some contexts but that also has oncosuppressive-like activity in PTEN-null tumors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9910 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human MCF-7 whole cell
IHC: human intestinal cancer tissue
ICC/IF: A431 cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ZBTB7A using anti-ZBTB7A antibody (PB9910).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB7A antigen affinity purified polyclonal antibody (Catalog # PB9910) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB7A at approximately 72 kDa. The expected band size for ZBTB7A is at 61 kDa.
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Figure 2. IHC analysis of ZBTB7A using anti-ZBTB7A antibody (PB9910).
ZBTB7A was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZBTB7A Antibody (PB9910) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IF analysis of ZBTB7A using anti-ZBTB7A antibody (PB9910).
ZBTB7A was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ZBTB7A Antibody (PB9910) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 4. Flow Cytometry analysis of MCF-7 cells using anti-ZBTB7A antibody (PB9910).
Overlay histogram showing MCF-7 cells stained with PB9910 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZBTB7A Antibody (PB9910, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ZBTB7A (Source: Uniprot.org, NCBI)
Gene Name
ZBTB7A
Full Name
Zinc finger and BTB domain-containing protein 7A
Weight
61439 MW
Alternative Names
Zinc finger and BTB domain-containing protein 7A;Factor binding IST protein 1;FBI-1;Factor that binds to inducer of short transcripts protein 1;HIV-1 1st-binding protein 1;Leukemia/lymphoma-related factor;POZ and Krueppel erythroid myeloid ontogenic factor;POK erythroid myeloid ontogenic factor;Pokemon;TTF-I-interacting peptide 21;TIP21;Zinc finger protein 857A;ZBTB7A;FBI1, LRF, ZBTB7, ZNF857A; Zbtb7a|9030619K07Rik, 9130006G12Rik, AI452336, FBI-, FBI-1, L, Lrf, Pok, Pokemon, Zbtb7|zinc finger and BTB domain containing 7a|zinc finger and BTB domain-containing protein 7A|POK erythroid myeloid ontogenic factor|POZ and Krueppel erythroid myeloid ontogenic factor|leukemia/lymphoma related factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZBTB7A, check out the ZBTB7A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZBTB7A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZBTB7A Antibody Picoband® (PB9910)
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Customer Q&As
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7 Customer Q&As for Anti-ZBTB7A Antibody Picoband®
Question
Please see the WB image, lot number and protocol we used for brain pancreas using anti-ZBTB7A antibody PB9910. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-01
Question
Do you have a BSA free version of anti-ZBTB7A antibody PB9910 available?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ZBTB7A antibody PB9910 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain pancreas using anti-ZBTB7A antibody PB9910. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-07
Question
Does PB9910 anti-ZBTB7A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
You can see on the product datasheet, PB9910 anti-ZBTB7A antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-27
Question
Would anti-ZBTB7A antibody PB9910 work on primate IHC with buccal mucosa cell?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
Our lab technicians have not tested anti-ZBTB7A antibody PB9910 on primate. You can run a BLAST between primate and the immunogen sequence of anti-ZBTB7A antibody PB9910 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate buccal mucosa cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-05
Question
I was wanting to use your anti-ZBTB7A antibody for WB for human brain pancreas on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain pancreas identification?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
As indicated on the product datasheet, PB9910 anti-ZBTB7A antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-15
Question
Is a blocking peptide available for product anti-ZBTB7A antibody (PB9910)?
G. Brown
Verified customer
Asked: 2016-05-02
Answer
We do provide the blocking peptide for product anti-ZBTB7A antibody (PB9910). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-05-02
