Product Info Summary
SKU: | PB9273 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Von Willebrand Factor/VWF Antibody Picoband™
SKU/Catalog Number
PB9273
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Von Willebrand Factor/VWF Antibody Picoband™ catalog # PB9273. Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Von Willebrand Factor/VWF Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9273)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse VWF recombinant protein (Position: M1304-E1452).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9273 is reactive to VWF in Mouse, Rat
Applications
PB9273 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
309 kDa
Calculated molecular weight
309269 MW
Background of VWF
Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunofluorescence, 2μg/ml, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-VWF Picoband antibody, PB9273, Western blotting
All lanes: Anti VWF (PB9273) at 0.5ug/ml
WB: Recombinant Mouse VWF Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
Click image to see more details
Anti-VWF Picoband antibody, PB9273, Western blotting
All lanes: Anti VWF (PB9273) at 0.5ug/ml
WB: Mouse Lung Tissue Lysate at 50ug
Predicted bind size: 309KD
Observed bind size: 309KD
Click image to see more details
Anti-VWF Picoband antibody, PB9273, IHC(P)
IHC(P): Mouse Liver Tissue
Click image to see more details
Figure 4. IHC analysis of VWF using anti-VWF antibody (PB9273).
VWF was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VWF Antibody (PB9273) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of VWF using anti-VWF antibody (PB9273).
VWF was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-VWF Antibody (PB9273) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. IF analysis of VWF and alpha-Smooth Muscle Actin using anti-VWF antibody (PB9273) and anti-alpha-Smooth Muscle Actin antibody (MA1106).
VWF and alpha-Smooth Muscle Actin a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-VWF antibody (PB9273) and mouse anti-alpha-Smooth Muscle Actin Antibody (MA1106) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For VWF (Source: Uniprot.org, NCBI)
Gene Name
VWF
Full Name
von Willebrand factor
Weight
309269 MW
Alternative Names
coagulation factor VIII VWF; F8; F8VWF; von Willebrand factor; VWD; vWF VWF F8VWF, VWD von Willebrand factor von Willebrand factor|coagulation factor VIII VWF
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VWF, check out the VWF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VWF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Von Willebrand Factor/VWF Antibody Picoband™ (PB9273)
Hello CJ!
PB9273 has been cited in 37 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Calcium signaling is implicated in the diffuse axonal injury of brain stem
Construction of tissue‑engineered bone using a bioreactor and platelet‑rich plasma
Identification and Analysis of Differentially Expressed Genes in Human Saphenous Vein Endothelial Cells Overexpressing Domain-Containing mTOR-Interacting Protein (DEPTOR) by RNA-Seq
Sodium alginate-bioglass-encapsulated hAECs restore ovarian function in premature ovarian failure by stimulating angiogenic factor secretion
Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar model
Combining ECM Hydrogels of Cardiac Bioactivity with Stem Cells of High Cardiomyogenic Potential for Myocardial Repair
ANGIOTENSIN-CONVERTING ENZYME INHIBITOR CAPTOPRIL PREVENTS OLEIC ACID-INDUCED SEVERE ACUTE LUNG INJURY IN RATS
Resolvin D2 Limits Secondary Tissue Necrosis After Burn Wounds in Rats
Mesenchymal stem cells derived from bone marrow favor tumor cell growth in vivo
Comparison of phenotypes and transcriptomes of mouse skin-derived precursors and dermal mesenchymal stem cells
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Customer Q&As
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17 Customer Q&As for Anti-Von Willebrand Factor/VWF Antibody Picoband™
Question
We noticed the the protein size is different on those 2 pictures of WB results poseted on your webpage. https://www.bosterbio.com/anti-vwf-picoband-trade-antibody-pb9273-boster.html The theoretical molecular weight of VWF is 309 KDa. Could you please help to explain why you got 35 KDa protein band?
Verified customer
Asked: 2022-11-20
Answer
The 37KD is generated by testing recombinant protein with partial rather than full length sequence. The 309KD is MW of target protein in tissues.
Boster Scientific Support
Answered: 2022-11-21
Question
I would like to test anti-Von Willebrand Factor/VWF antibody PB9273 on rat lung for research purposes, then I may be interested in using anti-Von Willebrand Factor/VWF antibody PB9273 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The products we sell, including anti-Von Willebrand Factor/VWF antibody PB9273, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-17
Question
I was wanting to use your anti-Von Willebrand Factor/VWF antibody for IHC-P for rat lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat lung identification?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
You can see on the product datasheet, PB9273 anti-Von Willebrand Factor/VWF antibody has been tested for IHC-P, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-17
Question
Is this PB9273 anti-Von Willebrand Factor/VWF antibody reactive to the isotypes of VWF?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The immunogen of PB9273 anti-Von Willebrand Factor/VWF antibody is E.coli-derived mouse VWF recombinant protein (Position: M1304-E1452). . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Von Willebrand Factor/VWF antibody PB9273. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-28
Question
My lab would like using your anti-Von Willebrand Factor/VWF antibody for r-mmu-372708; p130cas linkage to mapk signaling for integrins studies. Has this antibody been tested with western blotting on liver tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Thank you for your inquiry. This PB9273 anti-Von Willebrand Factor/VWF antibody is validated on mouse lung tissue, tissue lysate, liver tissue. It is guaranteed to work for IHC-P, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-27
Question
We are currently using anti-Von Willebrand Factor/VWF antibody PB9273 for mouse tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
The anti-Von Willebrand Factor/VWF antibody (PB9273) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-23
Question
Is a blocking peptide available for product anti-Von Willebrand Factor/VWF antibody (PB9273)?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
We do provide the blocking peptide for product anti-Von Willebrand Factor/VWF antibody (PB9273). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-30
Question
Will PB9273 anti-Von Willebrand Factor/VWF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Gonzalez
Verified customer
Asked: 2019-05-22
Answer
You can see on the product datasheet, PB9273 anti-Von Willebrand Factor/VWF antibody as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-22
Question
We purchased anti-Von Willebrand Factor/VWF antibody for WB on umbilical vein endothelial cell a few months ago. I am using mouse, and We want to use the antibody for IHC-P next. We want examining umbilical vein endothelial cell as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
I took a look at the website and datasheets of our anti-Von Willebrand Factor/VWF antibody and it seems that PB9273 has been validated on mouse in both WB and IHC-P. Thus PB9273 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-15
Question
My question regarding product PB9273, anti-Von Willebrand Factor/VWF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9273 anti-Von Willebrand Factor/VWF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-17
Question
We have observed staining in rat plasma. Do you have any suggestions? Is anti-Von Willebrand Factor/VWF antibody supposed to stain plasma positively?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
Based on literature plasma does express VWF. Based on Uniprot.org, VWF is expressed in lung, umbilical vein endothelial cell, platelet, plasma, liver, among other tissues. Regarding which tissues have VWF expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Plasma, Pubmed ID: 14760718
Platelet, Pubmed ID: 12665801
Umbilical vein endothelial cell, Pubmed ID: 3495266
Boster Scientific Support
Answered: 2018-04-26
Question
Do you have a BSA free version of anti-Von Willebrand Factor/VWF antibody PB9273 available?
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Von Willebrand Factor/VWF antibody PB9273 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-15
Question
I see that the anti-Von Willebrand Factor/VWF antibody PB9273 works with IHC-P, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-05-16
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-05-16
Question
See below the WB image, lot number and protocol we used for lung using anti-Von Willebrand Factor/VWF antibody PB9273. Please let me know if you require anything else.
B. Parker
Verified customer
Asked: 2016-08-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-09
Question
Does anti-Von Willebrand Factor/VWF antibody PB9273 work for IHC-P with lung?
S. Jones
Verified customer
Asked: 2016-07-13
Answer
According to the expression profile of lung, VWF is highly expressed in lung. So, it is likely that anti-Von Willebrand Factor/VWF antibody PB9273 will work for IHC-P with lung.
Boster Scientific Support
Answered: 2016-07-13
Question
My boss were content with the WB result of your anti-Von Willebrand Factor/VWF antibody. However we have seen positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is VWF supposed to be expressed?
P. Anderson
Verified customer
Asked: 2015-02-23
Answer
According to literature, liver does express VWF. Generally VWF expresses in secreted. Regarding which tissues have VWF expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Plasma, Pubmed ID: 14760718
Platelet, Pubmed ID: 12665801
Umbilical vein endothelial cell, Pubmed ID: 3495266
Boster Scientific Support
Answered: 2015-02-23