von Willebrand factor Antibodies

von Willebrand factor (VWF)

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also functions as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

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Gene Information

Human
Gene Name:	VWF
Uniprot:	P04275
Entrez:	7450

Family

Belongs to:
No superfamily

Alternative Names

coagulation factor VIII VWF; F8; F8VWF; von Willebrand factor; VWD; vWF

Molecular Weight

Mass (kDA):
309.265 kDA

Genomic Context

Human
Location:	12p13.31
Sequence:	12; NC_000012.12 (5948877..6124670, complement)

Tissue Specificity

Plasma.

Subcellular Localizations

Secreted. Secreted, extracellular space, extracellular matrix. Localized to storage granules.

Diseases

• Von Willebrand Disease
• Hemorrhage
• Tissue Adhesions
• Thrombosis
• Bleeding Tendency
• Purpura, Thrombotic Thrombocytopenic
• Purpura
• Thrombocytopenic Purpura
• Hemophilia A
• Thrombus
• Inflammation
• Raynaud Disease
• Blood Coagulation Disorders

• Von Willebrand Disease, Type 1
• Vascular System Injuries
• Von Willebrand Disease, Type 3
• Cardiovascular Diseases
• Vibration Syndrome
• Infarction
• Neoplasms

Interacting Proteins

• ADAMTS13
• F8
• GP1BA
• OPTN

Pathways

• Coagulation
• Platelet Aggregation
• Hemostasis
• Secretion
• Proteolysis
• Pathogenesis
• Platelet Activation
• Angiogenesis
• Exocytosis
• Fibrinolysis
• Localization
• Vasoconstriction
• Cell Adhesion

• Cell Activation
• Tolerance Induction
• Endothelial Cell Activation
• Blood Coagulation
• Glycosylation
• Aging
• Response To Cold

PTMs

• Cleavage
• Phosphorylation
• Glycosylation
• Reduction
• Oxidation
• Dephosphorylation
• Biotinylation
• Acetylation

• Methylation
• Sulphation
• Citrullination
• Ribosylation
• Nitration
• Deacetylation
• Palmitoylation
• Deglycosylation

• Myristoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/VWF

Best Uses Of The VWF Marker

Everybody has thought about how to utilize the VWF marker for cancer analysis. This article will teach you how to use the VWF Marker, and the benefits of this reagent. Boster pipettes permit you to dispense 0.5 milliliters through a one milliliter volume of aqueous solution. Multichannel pipettes are recommended when you need to use large quantities of samples. Sharing your findings with the scientific community can earn you product credits.

Multichannel pipettes are suggested for large amounts of samples.

In labs that study science multichannel pipettes can be found in science labs. They are electronic devices that measure and fill multiple vials with liquid. These devices are available in various sizes and styles, making pipetting much simpler and more precise. By pressing a button, multichannel pipettes will ensure the same level of liquid draw in each sample. This can reduce time and increase precision. It also allows scientists to get the most from their experiments. Which multichannel pipettes are the best? Let's find the answer.

In terms of ergonomics, multichannel pipettes are more comfortable to use over long durations of time. Because they have more channels and a wider surface area multichannel pipettes are better to handle large quantities of samples. Multichannel pipettes can also be used to dispense and aspirate into smaller containers. Multichannel pipettes come with a restricted design that can cause problems with handling and quality. To reduce strain and fatigue, the pipettes should be ergonomically designed.

Different tasks require different kinds of pipettes. Scientists have a wide range of choices when it comes to selecting pipettes. While some labs prefer a particular brand, it's crucial to think about all options before choosing one. Go to the Biocompare website to read about the reviews written by researchers in industry, government, and academia. This website lets scientists select the best pipettes for their research.

The Eppendorf Research Plus pipettes offer secondary adjustment calibration as well as an improved display of volume in four digits with magnifying feature. Multichannel pipettes are much easier to disinfect. The Eppendorf Research Plus pipettes feature Fortron(tm) polymer that is resistant to heat, mildew, and bleaching. They are also easy to clean with the PerfectPiston piston system. So, if you have a large amount of samples to analyze, a multichannel pipettes is the best option for you.

If you're looking to make use of a multichannel pipette to collect large samples, think about purchasing one with spring-loaded tip cones to reduce the risk of wrist and hand strain. They also have the smallest weight and force of operation when relative to other pipettes. The Physiocare Concept has been used to create the Eppendorf Research Plus pipette. The Physiocare Concept is a brand that stands for perfection in balance and durability. It has been integrated into this pipette. The pipette has been fully autoclavable, allowing for minimal user exertion and autoclaved without disassembling.

To ensure the accuracy of the results multichannel pipettes are used to test multiple sample dilutions. A 1:5 ratio should be used in columns two and four. Columns three and five should be the dilution 1:20. To make room for the sample, ensure that you calculate the amount of each column. Multichannel pipettes are ideal for handling large quantities of samples since they can eliminate repetitive motions, and allow researchers to produce more precise results.

Assays for enzyme-linked immunosorbents

Popular biochemistry instruments include enzyme-linked-immunosorbent tests (ELISAs). They identify a specific component in a sample typically blood samples or other biological fluid. The procedure uses an enzyme to bind the target antigen and a detection system to identify the signal. The manufacturers of ELISAs are Boster Bio, Expedeon, and RayBiotech.

Boster Bio enzyme-linked immunesorbent (ELISA), assays can detect various pathogens in water, food or air samples. These tests using enzymes are fast and simple to use and are extensively used in research laboratories and diagnostic laboratories around the world. While ELISA is an effective tool to detect numerous pathogens, there are still a lot of unknowns.

Enzyme-linked immunesorbent tests can detect proteins, peptides and hormones in samples. They are plate-based and extremely sensitive. The test involves putting an antigen on a surface, complexing it with an enzyme, then determining its activity by incubating the substrate. The product that is produced is quantifiable. The enzyme-linked immunosorbent assays are able to yield three types of data that include qualitative results, quantitative results, and a variety of applications that are clinically relevant.

Boster Bio's multiplex ELISA kits can measure up to 18 different analytes simultaneously. It requires 25 microliters of sample and costs up to 30 percent less than a standard multiplex assay. IQELISA(tm) is a method that combines ultrasensitive DNA amplification and the ELISA method, improves the sensitivity of detection and precision. IQELISAs are also affordable and can be utilized for research.

The direct ELISA employs only one antibody, which eliminates issues of cross-reactivity with the secondary antibody. The indirect method allows high-purity samples to directly coat the polystyrene plate, increasing the sensitivity and amplification of the signal. Indirect detection can detect proteins that target the sample at lower levels. These assays are ideal for research involving many biological samples, such as human organs and tissues.

One of the Boster Bio enzyme-linked immunosorbent-assays is an effective ELISA that detects 8-OHdG across various sample matrixes. It uses a specialized plate coated with mat KLK9 that has a biotinylated antiserum. This kit can detect DNA-incorporated and free 8-OHdG.

BosterBio's thorough IHC protocol

BosterBio's detailed IHC protocol unlike other kits includes an endogenous tissue background control (ECB). This is a tissue section that was not stained by the primary antibody. Background tissue from the endogenous source is crucial because certain tissues have fluorescent molecules that may confuse the results. BosterBio's protocol includes an ECB that lets you identify and remove the background that is created by the endogenous process.

The IHC scoring method comes with many pitfalls, including the possibility of background staining, that can cause mistakes in interpretation of results. Background staining may lead to mistakes in interpretation of results due to the fact that a lot of BMPs are expressed in osteoid tissue. A scoring system is recommended to prevent this issue. However it is not required in all situations.

A successful IHC procedure should have the correct controls. You should first include a negative control. Ensure that the sample contains no primary antibody. The negative control will show non-specific staining caused by secondary reactants. Furthermore, additional controls such as isotype and tissue type controls, can be useful for proving the specificity of the primary antibody. Finally, sample preparation is critical to the success of the IHC assay. The quality of the tissue sample determines how well the antigen will be detected.

There are a variety of ways to obtain tissue samples. Biopsy, surgery, and animal model can offer fresh tissues. Autopsy is a different method. Autopsy involves the removal of tissue from an animal within two hours of its death. The autopsy specimen is essentially postmortem autolysis and the antigens may have denatured by that time. To preserve the antigens it is crucial to adhere to the procedure as closely as is feasible.

To prevent binding that is non-specific blocking must be performed correctly , as with any IHC procedure. Additionally, blocking should be done using serum from the same species as the primary antibody. Autoantibody serum from animals can bind to previously blocked sites. Blocking should not be prolonged and should be done for between 10 and 30 minutes. If the antigen is not soluble the antigen should be removed prior to the second IHC procedure.

After you have removed the antigen, it's time to select the appropriate enzymatic substrate. The selection of a chromogen is also vital. There are many kinds of chromogens to choose from for each detection enzyme. HRP-DAB is the most popular combination. You can also make use of DAPI when HRP-DAB isn't available. This should not affect the signal of the label of the reporter.