Click image to see more details
Product Info Summary
| SKU: | M00270-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-VWF Antibody
SKU/Catalog Number
M00270-2
Size
80 µl
Description
Boster Bio Anti-VWF Antibody (Catalog # M00270-2). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-VWF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00270-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
907CT12.1.9
Isotype
IgG1
Immunogen
This antibody is generated from a mouse immunized with .VWF recombinant protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00270-2 is reactive to VWF in Human
Applications
M00270-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
309265 Da
Background of VWF
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1：2000
IHC-P: 1:25
FC: 1:100
Validation Images & Assay Conditions
Click image to see more details
Anti-VWF at dilution + VWF whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 309 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded H. kidney section using VWF. M00270-2 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Click image to see more details
Flow cytometric analysis of K562 cells using VWF(green, Cat#M00270-2) compared to an isotype control of mouse IgG1 (blue). HBG2 Antibody was diluted at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For VWF (Source: Uniprot.org, NCBI)
Gene Name
VWF
Full Name
von Willebrand factor
Weight
309265 Da
Alternative Names
coagulation factor VIII VWF; F8; F8VWF; von Willebrand factor; VWD; vWF VWF F8VWF, VWD von Willebrand factor von Willebrand factor|coagulation factor VIII VWF
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VWF, check out the VWF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VWF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VWF Antibody (M00270-2)
Hello CJ!
M00270-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li X,Zhou J,Dou Y,Shi Y,Wang Y,Hong J,Zhao J,Zhang J,Yuan Y,Zhou M,Wei X.The protective effects of angelica organic acid against ox-LDL-induced autophagy dysfunction of HUVECs.BMC Complement Med Ther.2020 Jun 1;20(1):164.doi:10.1186/s12906-020-02968-7.PMID:32487223;PMCID:PMC7268640.
Species: Human
M00270-2 usage in article: APP:IF, SAMPLE:HUVECS, DILUTION:1:50
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-VWF Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-VWF Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
