Anti-CD80 Antibody Picoband®

B7-1/CD80 antibody

Boster Bio Anti-CD80 Antibody catalog # PA1365-1. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: PA1365-1
Size: 100 μg/vial
Reactive Species: Rat
Host: Rabbit
Application: WB

Product Name

Anti-CD80 Antibody Picoband®

View all B7-1/CD80 Antibodies

SKU/Catalog Number

PA1365-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD80 Antibody catalog # PA1365-1. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD80 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1365-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80, different from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1365-1 is reactive to CD80 in Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

50 kDa

Calculated molecular weight

36298 MW

Background of B7-1/CD80

Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al. (1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1365-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat

Positive Control

WB: Rat Thymus Tissue

Validation Images & Assay Conditions

Gene/Protein Information For CD80 (Source: Uniprot.org, NCBI)

Gene Name

CD80

Full Name

T-lymphocyte activation antigen CD80

Weight

36298 MW

Alternative Names

CD80 ; CD80 B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7 CD80 molecule T-lymphocyte activation CD80|B-lymphocyte activation B7|CD80 (CD28 ligand 1, B7-1 )|CTLA-4 counter-receptor B7.1|activation B7-1 |costimulatory factor CD80|costimulatory molecule variant IgV-CD80

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD80, check out the CD80 Infographic

CD80 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD80: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1365-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Du Hy, Dong Lh, Zhao Bj, Fu J, Wang Qq, Chen F, Ou L, Li N, Sun X, Tang Zm, Song Hf. Acta Pharmacol Sin. 2012 Aug;33(8):1047-54. Doi: 10.1038/Aps.2012.54. Epub 2012 Jun 25. Immunostimulatory And Anti-Neoplasm Effects Of A Novel Palindrome Cpg Olig...

Have you used Anti-CD80 Antibody Picoband®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-CD80 Antibody Picoband®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-CD80 Antibody Picoband®

Question

Will PA1365-1 anti-CD80 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

It shows on the product datasheet, PA1365-1 anti-CD80 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-22

Question

We are currently using anti-CD80 antibody PA1365-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

The anti-CD80 antibody (PA1365-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-11

Question

Does anti-CD80 antibody PA1365-1 work for WB with gastrocnemius?

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

According to the expression profile of gastrocnemius, CD80 is highly expressed in gastrocnemius. So, it is likely that anti-CD80 antibody PA1365-1 will work for WB with gastrocnemius.

Boster Scientific Support

Answered: 2020-02-21

Question

I see that the anti-CD80 antibody PA1365-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-23

Question

Is there a BSA free version of anti-CD80 antibody PA1365-1 available?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD80 antibody PA1365-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-24

Question

I am looking for to test anti-CD80 antibody PA1365-1 on rat gastrocnemius for research purposes, then I may be interested in using anti-CD80 antibody PA1365-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

S. Miller

Verified customer

Asked: 2019-10-18

Answer

The products we sell, including anti-CD80 antibody PA1365-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-18

Question

Is this PA1365-1 anti-CD80 antibody reactive to the isotypes of CD80?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

The immunogen of PA1365-1 anti-CD80 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80(227-245aa DAHVSQNFTWEKPPEDPPD), different from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-27

Question

I was wanting to use your anti-CD80 antibody for WB for rat gastrocnemius on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat gastrocnemius identification?

Verified Customer

Verified customer

Asked: 2018-12-06

Answer

You can see on the product datasheet, PA1365-1 anti-CD80 antibody has been tested for WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat gastrocnemius in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-12-06

Question

Can you help my question with product PA1365-1, anti-CD80 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Kulkarni

Verified customer

Asked: 2018-11-28

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1365-1 anti-CD80 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-11-28

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for gastrocnemius using anti-CD80 antibody PA1365-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-06-25

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-25

Question

Is a blocking peptide available for product anti-CD80 antibody (PA1365-1)?

Verified Customer

Verified customer

Asked: 2018-06-07

Answer

We do provide the blocking peptide for product anti-CD80 antibody (PA1365-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-06-07

Question

Here is the WB image, lot number and protocol we used for gastrocnemius using anti-CD80 antibody PA1365-1. Please let me know if you require anything else.

D. Patel

Verified customer

Asked: 2017-07-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-05

Question

We have been able to see staining in rat gastrocnemius. What should we do? Is anti-CD80 antibody supposed to stain gastrocnemius positively?

R. Collins

Verified customer

Asked: 2015-06-11

Answer

According to literature gastrocnemius does express CD80. According to Uniprot.org, CD80 is expressed in gastrocnemius, lymphoid tissue, brain, erythroleukemia, among other tissues. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173

Boster Scientific Support

Answered: 2015-06-11

Question

Our team were content with the WB result of your anti-CD80 antibody. However we have seen positive staining in lymphoid tissue membrane using this antibody. Is that expected? Could you tell me where is CD80 supposed to be expressed?

J. Johnson

Verified customer

Asked: 2014-01-06

Answer

Based on literature, lymphoid tissue does express CD80. Generally CD80 expresses in membrane. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173

Boster Scientific Support

Answered: 2014-01-06

Question

Does anti-CD80 antibody PA1365-1 work on goat WB with lymphoid tissue?

M. Anderson

Verified customer

Asked: 2013-08-30

Answer

Our lab technicians have not validated anti-CD80 antibody PA1365-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CD80 antibody PA1365-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat lymphoid tissue in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-08-30

Order DetailsPrice
PA1365-1

100μg

$370
PA1365-1-10ug

10μg sample (liquid)

$99
PA1365-1-Biotin

100 μg Biotin conjugated

$570
PA1365-1-Cy3

100 μg Cy3 conjugated

$570
PA1365-1-Dylight488

100 μg Dylight488 conjugated

$570
PA1365-1-Dylight550

100 μg Dylight550 conjugated

$570
PA1365-1-Dylight594

100 μg Dylight594 conjugated

$570
PA1365-1-FITC

100 μg FITC conjugated

$570
PA1365-1-HRP

100 μg HRP conjugated

$570
PA1365-1-APC

100 μg APC conjugated

$670
PA1365-1-PE

100 μg PE conjugated

$670
PA1365-1-iFluor647

100 μg iFluor647 conjugated

$670
PA1365-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
PA1365-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.