Click image to see more details
Product Info Summary
| SKU: | A07261-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL18 binding protein/Il18bp Antibody Picoband®
SKU/Catalog Number
A07261-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL18 binding protein/Il18bp Antibody Picoband® catalog # A07261-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL18 binding protein/Il18bp Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07261-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL18 binding protein recombinant protein (Position: T29-A193).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07261-1 is reactive to Il18bp in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
67166 MW
Background of IL18BP
Interleukin-18-binding protein is a protein that in humans is encoded by the IL18BP gene. The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07261-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: mouse heart tissue, mouse brain tissue, rat heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL18 binding protein using anti-IL18 binding protein antibody (A07261-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysate,
Lane 2: mouse brain tissue lysate,
Lane 3: rat heart tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 binding protein antigen affinity purified polyclonal antibody (Catalog # A07261-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 binding protein at approximately 43KD. The expected band size for IL18 binding protein is at 21KD.
Protein Target Info & Infographic
Gene/Protein Information For Il18bp (Source: Uniprot.org, NCBI)
Gene Name
Il18bp
Full Name
Interleukin-18-binding protein
Weight
67166 MW
Alternative Names
Interleukin-18-binding protein; IL-18BP; Interferon gamma-inducing factor-binding protein; Il18bp; Igifbp IL18BP FVHa, IL18BP interleukin 18 binding protein interleukin-18-binding protein|MC51L-53L-54L homolog gene product|tadekinig-alfa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il18bp, check out the Il18bp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il18bp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL18 binding protein/Il18bp Antibody Picoband® (A07261-1)
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Customer Q&As
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7 Customer Q&As for Anti-IL18 binding protein/Il18bp Antibody Picoband®
Question
Is there a BSA free version of anti-IL18 binding protein/Il18bp antibody A07261-1 available?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL18 binding protein/Il18bp antibody A07261-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-24
Question
My question regarding product A07261-1, anti-IL18 binding protein/Il18bp antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07261-1 anti-IL18 binding protein/Il18bp antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-27
Question
We are currently using anti-IL18 binding protein/Il18bp antibody A07261-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
The anti-IL18 binding protein/Il18bp antibody (A07261-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-19
Question
Is this A07261-1 anti-IL18 binding protein/Il18bp antibody reactive to the isotypes of IL18BP?
J. Evans
Verified customer
Asked: 2020-02-05
Answer
The immunogen of A07261-1 anti-IL18 binding protein/Il18bp antibody is E. coli-derived mouse IL18 binding protein recombinant protein (Position: T29-A193). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-05
Question
Would anti-IL18 binding protein/Il18bp antibody A07261-1 work on goat WB with t-cell?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
Our lab technicians have not validated anti-IL18 binding protein/Il18bp antibody A07261-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-IL18 binding protein/Il18bp antibody A07261-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-18
Question
Would A07261-1 anti-IL18 binding protein/Il18bp antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
You can see on the product datasheet, A07261-1 anti-IL18 binding protein/Il18bp antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-28
Question
Will anti-IL18 binding protein/Il18bp antibody A07261-1 work for WB with leukocyte?
L. Rodriguez
Verified customer
Asked: 2013-01-25
Answer
According to the expression profile of leukocyte, IL18BP is highly expressed in leukocyte. So, it is likely that anti-IL18 binding protein/Il18bp antibody A07261-1 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2013-01-25
