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Product Info Summary
| SKU: | PA1889 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Krueppel-like factor 5 KLF5 Antibody Picoband®
SKU/Catalog Number
PA1889
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Krueppel-like factor 5 KLF5 Antibody catalog # PA1889. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Krueppel-like factor 5 KLF5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1889)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human KLF5, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1889 is reactive to KLF5 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
50792 MW
Background of KLF5
KLF5 (Krueppel-like factor 5) is a protein that in humans is encoded by the KLF5 gene. It is also known as BASIC TRANSCRIPTION ELEMENT-BINDING PROTEIN 2 or BTEB2. This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Suske et al. (2005)stated that the human KLF5 gene maps to chromosome 13q21.33, and the mouse Klf5 gene to chromosome 14E2.1. Sogawa et al. (1993)isolated a cDNA encoding BTEB2. The predicted 219-amino acid protein contains 3 consecutive zinc finger motifs near the C terminus. The zinc finger domains were 59% and 64% identical to those of Sp1 (189906) and BTEB1, respectively. Both BTEB2 and Sp1 have a short basic region preceding the zinc finger motifs that may play an auxiliary role in DNA binding. The N-terminal region of BTEB2 is proline rich. Recombinant BTEB2 protein and Sp1 showed very similar DNA-binding specificity in vitro. When expressed in mammalian cells, BTEB2 activated the transcription of a reporter gene fused to a GC box-containing promoter. Sogawa et al. (1993) also identified the rat BTEB2 homolog. Northern blot analysis of rat tissues revealed that BTEB2 is expressed only in the testis and placenta.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1889 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human A549 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of KLF5 using anti-KLF5 antibody (PA1889).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF5 antigen affinity purified polyclonal antibody (Catalog # PA1889) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF5 at approximately 60 kDa. The expected band size for KLF5 is at 51 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KLF5 (Source: Uniprot.org, NCBI)
Gene Name
KLF5
Full Name
Krueppel-like factor 5
Weight
50792 MW
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Krueppel-like factor 5;Basic transcription element-binding protein 2;BTE-binding protein 2;Colon krueppel-like factor;GC-box-binding protein 2;Intestinal-enriched krueppel-like factor;Transcription factor BTEB2;KLF5;BTEB2, CKLF, IKLF; KLF5 BTEB2, CKLF, IKLF Kruppel like factor 5 Krueppel-like factor 5|(intestinal Kruppel-like factor|BTE-binding protein 2|GC box binding protein 2|Kruppel-like factor 5 (intestinal)|basic transcription element binding protein 2|colon krueppel-like factor|colon kruppel-like factor|epididymis secretory sperm binding protein|intestinal-enriched krueppel-like factor|intestinal-enriched kruppel-like factor|transcription factor BTEB2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF5, check out the KLF5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Krueppel-like factor 5 KLF5 Antibody Picoband® (PA1889)
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Customer Q&As
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5 Customer Q&As for Anti-Krueppel-like factor 5 KLF5 Antibody Picoband®
Question
I have a question about product PA1889, anti-KLF5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1889 anti-KLF5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-08
Question
Is this PA1889 anti-KLF5 antibody reactive to the isotypes of KLF5?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The immunogen of PA1889 anti-KLF5 antibody is A synthetic peptide corresponding to a sequence in the middle region of human KLF5(109-126aa HKKYRRDSASVVDQFFTD), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-22
Question
My lab would like to test anti-KLF5 antibody PA1889 on rat testis for research purposes, then I may be interested in using anti-KLF5 antibody PA1889 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The products we sell, including anti-KLF5 antibody PA1889, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-27
Question
We are currently using anti-KLF5 antibody PA1889 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
The anti-KLF5 antibody (PA1889) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-30
Question
Would anti-KLF5 antibody PA1889 work on monkey WB with brain placenta?
Verified Customer
Verified customer
Asked: 2018-11-20
Answer
Our lab technicians have not validated anti-KLF5 antibody PA1889 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-KLF5 antibody PA1889 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-20
