Click image to see more details
Product Info Summary
| SKU: | M00270-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody
SKU/Catalog Number
M00270-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody catalog # M00270-1. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00270-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IHF-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human VWF
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00270-1 is reactive to VWF in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated molecular weight
309265 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00270-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human serum
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of VWF expression in human serum lysate.
Protein Target Info & Infographic
Gene/Protein Information For VWF (Source: Uniprot.org, NCBI)
Gene Name
VWF
Full Name
von Willebrand factor
Weight
309265 MW
Alternative Names
von Willebrand factor;vWF;von Willebrand antigen 2;von Willebrand antigen II;VWF;F8VWF; VWF F8VWF, VWD von Willebrand factor von Willebrand factor|coagulation factor VIII VWF
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VWF, check out the VWF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VWF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody (M00270-1)
Hello CJ!
M00270-1 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Homocysteine induced oxidative stress in human umbilical vein endothelial cells via regulating methylation of SORBS1.
Protein transduction domain-hA20 fusion protein protects endothelial cells against high glucose-induced injury
Resveratrol Protects against TNF-?-Induced Injury in Human Umbilical Endothelial Cells through Promoting Sirtuin-1-Induced Repression of NF-KB and p38 MAPK
IFI6 Inhibits Apoptosis via Mitochondrial-Dependent Pathway in Dengue Virus 2 Infected Vascular Endothelial Cells
Paxillin regulates vascular endothelial growth factor A-induced?in vitro?angiogenesis of human umbilical vein endothelial cells
Yan J, Wang J, Huang H, Huang Y, Mi T, Zhang C, Zhang L. Am J Transl Res. 2017 Oct 15;9(10):4492-4501. eCollection 2017. Fibroblast growth factor 21 delayed endothelial replicative senescence and protected cells from H2O2-induced premature senesce...
Yang H, Wu S, Feng R, Huang J, Liu L, Liu F, Chen Y. Stem Cell Res Ther. 2017 Nov 21;8(1):267. doi: 10.1186/s13287-017-0718-8. Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats
Zhu Y, Tuerxun A, Hui Y, Abliz P. Exp Ther Med. 2014 Aug;8(2):647-651. Epub 2014 Jun 12. Effects Of Propranolol And Isoproterenol On Infantile Hemangioma Endothelial Cells In Vitro.
Zhao Xy, Chen L, Li Yh, Xu Q. World J Gastroenterol. 2007 Dec 28;13(48):6558-61. Plexina1 Expression In Gastric Carcinoma And Its Relationship With Tumor Angiogenesis And Proliferation.
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Customer Q&As
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3 Customer Q&As for Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody
Question
My team were satisfied with the WB result of your anti-VWF/Von Willebrand Factor Rabbit Monoclonal antibody. However we have been able to see positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is VWF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
According to literature, liver does express VWF. Generally VWF expresses in secreted. Regarding which tissues have VWF expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Plasma, Pubmed ID: 14760718
Platelet, Pubmed ID: 12665801
Umbilical vein endothelial cell, Pubmed ID: 3495266
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-VWF/Von Willebrand Factor Rabbit Monoclonal antibody M00270-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
The anti-VWF/Von Willebrand Factor Rabbit Monoclonal antibody (M00270-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-23
Question
We have observed staining in human liver. Are there any suggestions? Is anti-VWF/Von Willebrand Factor Rabbit Monoclonal antibody supposed to stain liver positively?
D. Miller
Verified customer
Asked: 2016-04-27
Answer
From literature liver does express VWF. From Uniprot.org, VWF is expressed in lung, umbilical vein endothelial cell, platelet, plasma, liver, among other tissues. Regarding which tissues have VWF expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Plasma, Pubmed ID: 14760718
Platelet, Pubmed ID: 12665801
Umbilical vein endothelial cell, Pubmed ID: 3495266
Boster Scientific Support
Answered: 2016-04-27
