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Product Info Summary
| SKU: | PB9489 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CIB1 Antibody Picoband®
SKU/Catalog Number
PB9489
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CIB1 Antibody Picoband® catalog # PB9489. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CIB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9489)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CIB1 recombinant protein (Position: S6-L191). Human CIB1 shares 93.5% amino acid (aa) sequence identity with both mouse and rat CIB1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9489 is reactive to CIB1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
27 kDa
Calculated molecular weight
21703 MW
Background of CIB1
Calcium and integrin-binding protein 1 is a protein that in humans is encoded by the CIB1 gene. This gene encodes a member of the EF-hand domain-containing calcium-binding superfamily. And this protein interacts with many other proteins, including the platelet integrin alpha-IIb-beta-3, DNA-dependent protein kinase, presenilin-2, focal adhesion kinase, p21 activated kinase, and protein kinase D. Moreover, the encoded protein may be involved in cell survival and proliferation, and is associated with several disease states including cancer and Alzheimer's disease. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9489 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human A431 whole cell, rat PC-12 whole cell, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CIB1 using anti-CIB1 antibody (PB9489).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse brian tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIB1 antigen affinity purified polyclonal antibody (Catalog # PB9489) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIB1 at approximately 22 kDa. The expected band size for CIB1 is at 27 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CIB1 (Source: Uniprot.org, NCBI)
Gene Name
CIB1
Full Name
Calcium and integrin-binding protein 1
Weight
21703 MW
Alternative Names
Calcium and integrin-binding protein 1;CIB;Calcium- and integrin-binding protein;CIBP;Calmyrin;DNA-PKcs-interacting protein;Kinase-interacting protein;KIP;SNK-interacting protein 2-28;SIP2-28;CIB1;CIB, KIP, PRKDCIP; CIB1 CIB, CIBP, KIP1, PRKDCIP, SIP2-28 calcium and integrin binding 1 calcium and integrin-binding protein 1|DNA-PK interaction protein|DNA-PKcs-interacting protein|DNA-dependent protein kinase interacting protein|SNK-interacting protein 2-28|calcium and integrin binding 1 (calmyrin)|testicular secretory protein Li 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CIB1, check out the CIB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CIB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CIB1 Antibody Picoband® (PB9489)
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1 Customer Q&As for Anti-CIB1 Antibody Picoband®
Question
We are currently using anti-CIB1 antibody PB9489 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
K. Evans
Verified customer
Asked: 2018-05-01
Answer
The anti-CIB1 antibody (PB9489) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-01
