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Product Info Summary
| SKU: | PA1365 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD80 Antibody Picoband®
SKU/Catalog Number
PA1365
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD80 Antibody catalog # PA1365. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD80 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1365)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CD80.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1365 is reactive to CD80 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
33048 MW
Background of B7-1/CD80
Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al. (1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1365 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Raji whole cell, human Daudi whole cell, human Ramos whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD80 using anti-CD80 antibody (PA1365).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human Ramos whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD80 antigen affinity purified polyclonal antibody (Catalog # PA1365) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD80 at approximately 70 kDa. The expected band size for CD80 is at 33 kDa.
Click image to see more details
Figure 2. Western blot analysis of CD80 using anti-CD80 antibody (PA1549).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD80 antigen affinity purified polyclonal antibody (Catalog # PA1549) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD80 approximately 70 kDa. The expected band size for CD80 is at 33 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CD80 (Source: Uniprot.org, NCBI)
Gene Name
CD80
Full Name
T-lymphocyte activation antigen CD80
Weight
33048 MW
Alternative Names
T-lymphocyte activation antigen CD80;Activation B7-1 antigen;BB1;CTLA-4 counter-receptor B7.1;B7;CD80;CD80;CD28LG, CD28LG1, LAB7; CD80 B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7 CD80 molecule T-lymphocyte activation CD80|B-lymphocyte activation B7|CD80 (CD28 ligand 1, B7-1 )|CTLA-4 counter-receptor B7.1|activation B7-1 |costimulatory factor CD80|costimulatory molecule variant IgV-CD80
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD80, check out the CD80 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD80: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD80 Antibody Picoband® (PA1365)
Hello CJ!
PA1365 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Du Hy, Dong Lh, Zhao Bj, Fu J, Wang Qq, Chen F, Ou L, Li N, Sun X, Tang Zm, Song Hf. Acta Pharmacol Sin. 2012 Aug;33(8):1047-54. Doi: 10.1038/Aps.2012.54. Epub 2012 Jun 25. Immunostimulatory And Anti-Neoplasm Effects Of A Novel Palindrome Cpg Olig...
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-CD80 Antibody Picoband®
Question
Can you help my question with product PA1365, anti-CD80 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Yang
Verified customer
Asked: 2020-03-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1365 anti-CD80 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-09
Question
Is this PA1365 anti-CD80 antibody reactive to the isotypes of CD80?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
The immunogen of PA1365 anti-CD80 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CD80(57-71aa EELAQTRIYWQKEKK). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-05
Question
We are currently using anti-CD80 antibody PA1365 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
C. Zhao
Verified customer
Asked: 2020-02-03
Answer
The anti-CD80 antibody (PA1365) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-03
Question
Our team were well pleased with the WB result of your anti-CD80 antibody. However we have seen positive staining in erythroleukemia membrane using this antibody. Is that expected? Could you tell me where is CD80 supposed to be expressed?
R. Mangal
Verified customer
Asked: 2020-01-29
Answer
Based on literature, erythroleukemia does express CD80. Generally CD80 expresses in membrane. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173
Boster Scientific Support
Answered: 2020-01-29
Question
Does anti-CD80 antibody PA1365 work for WB with lymphoid tissue?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
According to the expression profile of lymphoid tissue, CD80 is highly expressed in lymphoid tissue. So, it is likely that anti-CD80 antibody PA1365 will work for WB with lymphoid tissue.
Boster Scientific Support
Answered: 2019-12-12
Question
Please see the WB image, lot number and protocol we used for lymphoid tissue using anti-CD80 antibody PA1365. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-18
Question
Is a blocking peptide available for product anti-CD80 antibody (PA1365)?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
We do provide the blocking peptide for product anti-CD80 antibody (PA1365). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-11
Question
We have been able to see staining in human erythroleukemia. Are there any suggestions? Is anti-CD80 antibody supposed to stain erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
From literature erythroleukemia does express CD80. From Uniprot.org, CD80 is expressed in gastrocnemius, lymphoid tissue, brain, erythroleukemia, among other tissues. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173
Boster Scientific Support
Answered: 2019-10-25
Question
Would PA1365 anti-CD80 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
You can see on the product datasheet, PA1365 anti-CD80 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-19
Question
you antibody to test anti-CD80 antibody PA1365 on human lymphoid tissue for research purposes, then I may be interested in using anti-CD80 antibody PA1365 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The products we sell, including anti-CD80 antibody PA1365, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-02
Question
Is there a BSA free version of anti-CD80 antibody PA1365 available?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD80 antibody PA1365 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-22
Question
I was wanting to use your anti-CD80 antibody for WB for human lymphoid tissue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphoid tissue identification?
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
You can see on the product datasheet, PA1365 anti-CD80 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymphoid tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-09
Question
I see that the anti-CD80 antibody PA1365 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphoid tissue using anti-CD80 antibody PA1365. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-29
