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Product Info Summary
| SKU: | A00618-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MR1 Antibody Picoband®
SKU/Catalog Number
A00618-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MR1 Antibody catalog # A00618-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00618-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MR1 recombinant protein (Position: R23-D269).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00618-1 is reactive to MR1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
57371 MW
Background of MR1
Major histocompatibility complex class I-related gene protein is a protein that in humans is encoded by the MR1 gene. MAIT (mucosal-associated invariant T-cells) lymphocytes represent a small population of T-cells primarily found in the gut. The protein encoded by this gene is an antigen-presenting molecule that presents metabolites of microbial vitamin B to MAITs. This presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. Several transcript variants encoding different isoforms have been found for this gene, and a pseudogene of it has been detected about 36 kbp upstream on the same chromosome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00618-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human T-47D whole cell, human U-937 whole cell, human A431 whole cell
FCM: SiHa cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MR1 using anti-MR1 antibody (A00618-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human T-47D whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MR1 antigen affinity purified polyclonal antibody (Catalog # A00618-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MR1 at approximately 40KD. The expected band size for MR1 is at 43KD.
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Figure 2. Flow Cytometry analysis of SiHa cells using anti-MR1 antibody (A00618-1).
Overlay histogram showing SiHa cells stained with A00618-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MR1 Antibody (A00618-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MR1 (Source: Uniprot.org, NCBI)
Gene Name
MR1
Full Name
Major histocompatibility complex class I-related gene protein
Weight
57371 MW
Superfamily
MHC class I family
Alternative Names
Major histocompatibility complex class I-related gene protein; MHC class I-related gene protein; Class I histocompatibility antigen-like protein; MR1 MR1 HLALS major histocompatibility complex, class I-related major histocompatibility complex class I-related gene protein|MHC class I-like MR-1|MHC class-I related-gene protein|major histocompatibility complex, class I-like sequence
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MR1, check out the MR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MR1 Antibody Picoband® (A00618-1)
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Customer Q&As
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3 Customer Q&As for Anti-MR1 Antibody Picoband®
Question
Does anti-MR1 antibody A00618-1 work for Flow Cytometry with placenta?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
According to the expression profile of placenta, MR1 is highly expressed in placenta. So, it is likely that anti-MR1 antibody A00618-1 will work for Flow Cytometry with placenta.
Boster Scientific Support
Answered: 2020-04-02
Question
I was wanting to use your anti-MR1 antibody for Flow Cytometry for mouse placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
You can see on the product datasheet, A00618-1 anti-MR1 antibody has been tested for ELISA, Flow Cytometry, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-09
Question
We are currently using anti-MR1 antibody A00618-1 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
T. Carter
Verified customer
Asked: 2017-12-12
Answer
The anti-MR1 antibody (A00618-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-12
