Product Info Summary
SKU: | A00270-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC |
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Product info
Product Name
Anti-Von Willebrand Factor/VWF Antibody Picoband™
SKU/Catalog Number
A00270-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Von Willebrand Factor/VWF Antibody Picoband™ catalog # A00270-1. Tested in ELISA, IHC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Von Willebrand Factor/VWF Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00270-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Von Willebrand Factor/VWF recombinant protein (Position: R1287-Q2770).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00270-1 is reactive to VWF in Human
Applications
A00270-1 is guaranteed for ELISA, IHC Boster Guarantee
Observed Molecular Weight
49 kDa
Calculated molecular weight
68762 MW
Background of VWF
Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1).
Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1).
Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1).
Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For VWF (Source: Uniprot.org, NCBI)
Gene Name
VWF
Full Name
von Willebrand factor
Weight
68762 MW
Alternative Names
coagulation factor VIII VWF; F8; F8VWF; von Willebrand factor; VWD; vWF VWF F8VWF, VWD von Willebrand factor von Willebrand factor|coagulation factor VIII VWF
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VWF, check out the VWF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VWF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Von Willebrand Factor/VWF Antibody Picoband™ (A00270-1)
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