Anti-FOXA1 / HNF3A Monoclonal Antibody

Product Name

Anti-FOXA1 / HNF3A Monoclonal Antibody

View all HNF-3 alpha/FoxA1 Antibodies

SKU/Catalog Number

M00266

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-FOXA1 / HNF3A Monoclonal Antibody (Catalog # M00266). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-FOXA1 / HNF3A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00266)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: FOXA1/1515

Isotype

IgG1, kappa

Immunogen

Recombinant human FOXA1 protein fragment (aa372-472) (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00266 is reactive to FOXA1 in Human

Applications

M00266 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

49148 MW

Background of HNF-3 alpha/FoxA1

The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors.HNF-1 (), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Anti-FOXA1 Mouse Monoclonal Antibody (FOXA1/1515).

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Anti-FOXA1 Mouse Monoclonal Antibody (FOXA1/1515).

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Western Blot analysis of HeLa cell lysate using Anti-FOXA1 Mouse Monoclonal Antibody (FOXA1/1515).

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Flow Cytometric analysis of PFA fixed MCF-7 cells using Anti-FOXA1 Mouse Monoclonal Antibody (FOXA1/1515); followed by goat anti-mouse IgG-CF488 (Blue); Goat anti-mouse IgG-CF488 Is Control (Red).

Gene/Protein Information For FOXA1 (Source: Uniprot.org, NCBI)

Alternative Names

forkhead box A1; Forkhead box protein A1; FoxA1; hepatocyte nuclear factor 3, alpha; hepatocyte nuclear factor 3-alpha; HNF3 alpha; HNF-3 alpha; HNF-3A; HNF-3-alpha; HNF3ATCF-3A; MGC33105; TCF3A; Transcription factor 3A FOXA1 HNF3A, TCF3A forkhead box A1 hepatocyte nuclear factor 3-alpha|HNF-3-alpha|HNF-3A|TCF-3A|forkhead box protein A1|transcription factor 3A

For more info on FOXA1, check out the FOXA1 Infographic

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In this infographic, you will see the following information for FOXA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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