Product Info Summary
SKU: | M01893-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PP2A-alpha/PPP2CA Antibody Picoband™ (monoclonal, 3B6)
View all PP2A alpha Antibodies
SKU/Catalog Number
M01893-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PP2A-alpha/PPP2CA Antibody Picoband™ (monoclonal, 3B6) catalog # M01893-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PP2A-alpha/PPP2CA Antibody Picoband™ (monoclonal, 3B6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01893-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
3B6
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309). Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01893-1 is reactive to PPP2CA in Human, Monkey, Mouse, Rat
Applications
M01893-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
36 kDa
Calculated molecular weight
25035 MW
Background of PP2A alpha
The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 4μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
![m01893 1 pp2a1 primary antibodies wb testing 1 m01893 1 pp2a1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m01893-1-pp2a1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (M01893-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human HEPG2 whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PP2A-alpha/PPP2CA antigen affinity purified monoclonal antibody (Catalog # M01893-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PP2A-alpha/PPP2CA at approximately 36KD. The expected band size for PP2A-alpha/PPP2CA is at 36KD.
![m01893 1 pp2a1 primary antibodies if testing 2 m01893 1 pp2a1 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m01893-1-pp2a1-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (M01893-1).
PP2A-alpha/PPP2CA was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL mouse anti-PP2A-alpha/PPP2CA Antibody (M01893-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![m01893 1 pp2a1 primary antibodies fcm testing 3 m01893 1 pp2a1 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m01893-1-pp2a1-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of U937 cells using anti-PP2A-alpha/PPP2CA antibody (M01893-1).
Overlay histogram showing U937 cells stained with M01893-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PP2A-alpha/PPP2CA Antibody (M01893-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![m01893 1 pp2a1 primary antibodies ihc testing 4 m01893 1 pp2a1 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/m/0/m01893-1-pp2a1-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (M01893-1).
PP2A-alpha/PPP2CA was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PP2A-alpha/PPP2CA Antibody (M01893-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
![m01893 1 pp2a1 primary antibodies ihc testing 5 m01893 1 pp2a1 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/m/0/m01893-1-pp2a1-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (M01893-1).
PP2A-alpha/PPP2CA was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PP2A-alpha/PPP2CA Antibody (M01893-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
![m01893 1 pp2a1 primary antibodies ihc testing 6 m01893 1 pp2a1 primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/m/0/m01893-1-pp2a1-primary-antibodies-ihc-testing-6.jpg)
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Figure 6. IHC analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (M01893-1).
PP2A-alpha/PPP2CA was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PP2A-alpha/PPP2CA Antibody (M01893-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PPP2CA (Source: Uniprot.org, NCBI)
Gene Name
PPP2CA
Full Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Weight
25035 MW
Superfamily
PPP phosphatase family
Alternative Names
EC 3.1.3.16; PP2A; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform PPP2CA NEDLBA, PP2Ac, PP2CA, PP2Calpha, RP-C protein phosphatase 2 catalytic subunit alpha serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform|PP2A-alpha|protein phosphatase 2, catalytic subunit, alpha isozyme|replication protein C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP2CA, check out the PPP2CA Infographic
![PPP2CA infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP2CA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PP2A-alpha/PPP2CA Antibody Picoband™ (monoclonal, 3B6) (M01893-1)
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