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Product Info Summary
| SKU: | A02128 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PSD95/DLG4 Antibody Picoband®
SKU/Catalog Number
A02128
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PSD95/DLG4 Antibody Picoband® catalog # A02128. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PSD95/DLG4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02128)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PSD95/DLG4 recombinant protein (Position: D18-L711).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02128 is reactive to DLG4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
99 kDa
Calculated molecular weight
51990 MW
Background of PSD-95
DLG4 discs large homolog 4, also known as PSD95 or SAP-90, is a protein that in humans is encoded by the DLG4 gene. It is a member of the membrane-associated guanylate kinase (MAGUK) family. This gene is mapped to 17p13.1. DLG4 can heteromultimerize with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Overexpression of DLG4 in hippocampal neurons could drive maturation of glutamatergic synapses. DLG4 can orchestrate synaptic development and it has a role in synapse stabilization and plasticity. Ubiquitination of DLG4 through an MDM2-mediated pathway can regulate AMPA receptor surface expression during synaptic plasticity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02128 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
FCM: U937 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (A02128).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD95/DLG4 antigen affinity purified polyclonal antibody (Catalog # A02128) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD95/DLG4 at approximately 99KD. The expected band size for PSD95/DLG4 is at 99KD.
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Figure 2. Flow Cytometry analysis of U937 cells using anti-PSD95/DLG4 antibody (A02128).
Overlay histogram showing U937 cells stained with A02128 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PSD95/DLG4 Antibody (A02128, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For DLG4 (Source: Uniprot.org, NCBI)
Gene Name
DLG4
Full Name
Disks large homolog 4
Weight
51990 MW
Superfamily
MAGUK family
Alternative Names
Carnitine O-palmitoyltransferase 2, mitochondrial; Carnitine palmitoyltransferase II; CPT II; CPT2; CPT1 DLG4 MRD62, PSD95, SAP-90, SAP90 discs large MAGUK scaffold protein 4 disks large homolog 4|Tax interaction protein 15|discs large homolog 4|post-synaptic density protein 95|synapse-associated protein 90
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DLG4, check out the DLG4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DLG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSD95/DLG4 Antibody Picoband® (A02128)
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