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Product Info Summary
| SKU: | EKC2110 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
PP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2110
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The PP2A-alpha (Phospho-Tyr307) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PP2A-alpha (Phospho-Tyr307) protein expression profile in cells. The kit can be used for measuring the relative amounts of PP2A-alpha (Phospho-Tyr307) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PP2A-alpha (Phospho-Tyr307).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
PP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2110)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2110 is reactive to PPP2CA in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2110 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-PP2A-alpha Phospho-Tyr307 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-PP2A-alpha Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from A549 cells
Protein Target Info & Infographic
Gene/Protein Information For PPP2CA (Source: Uniprot.Org, NCBI)
Gene Name
PPP2CA
Full Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Weight
35594 MW
Superfamily
PPP phosphatase family
Alternative Names
PP2A-alpha;PP2AA;RP-C;Replication protein C;Serine/threonine protein phosphatase 2A;catalytic subunit, alpha isoform PPP2CA NEDLBA, PP2Ac, PP2CA, PP2Calpha, RP-C protein phosphatase 2 catalytic subunit alpha serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform|PP2A-alpha|protein phosphatase 2, catalytic subunit, alpha isozyme|replication protein C
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PPP2CA, check out the PPP2CA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PPP2CA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit (EKC2110)
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Customer Q&As
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1 Customer Q&As for PP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit
Question
Hello Support; I am working with filamentous fungi Neurospora crassa and I would like to order antibody for protein phosphatase PP2A catalytic subunit. Upon visiting your website I saw PP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit, but my concern is that these antibody will not link to Neurospora crassa protein PP2A as there are some differences in peptide sequence between human PP2A and Neurospora crassa PP2A catalytic subunit. I need your advice on this. Best regrads.
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
We normally expect a cross reaction between species of an antibody when the homology is 85% and higher and we consider it as worth testing. But, the immunogen of PP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit and Neurospora crassa shows only 78% homology. Thus, we cannot guarantee this antibody for your purposes.
Boster Scientific Support
Answered: 2019-01-02
