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Product Info Summary
| SKU: | PA1068 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PP2A-alpha/PPP2CA Antibody Picoband®
View all PP2A alpha Antibodies
SKU/Catalog Number
PA1068
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PP2A-alpha/PPP2CA Antibody catalog # PA1068. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PP2A-alpha/PPP2CA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1068)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PP2A-alpha, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1068 is reactive to PPP2CA in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
35594 MW
Background of PP2A alpha
The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide.It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1068 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Positive Control
WB: human Hela whole cell, human CACO-2 whole cell, human U251 whole cell, rat brain tissue, rat PC-12 whole cell, mouse lung tissue, mouse liver tissue, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: Human Mammary Cancer tissue, Rat Kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PP2A-alpha using anti-PP2A-alpha antibody (PA1068).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse brain tissue lysates,
Lane 9: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PP2A-alpha antigen affinity purified polyclonal antibody (Catalog # PA1068) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PP2A-alpha at approximately 36 kDa. The expected band size for PP2A-alpha is at 36 kDa.
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Figure 2. IHC analysis of PP2A-alpha using anti-PP2A-alpha antibody (PA1068).
PP2A-alpha was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PP2A-alpha Antibody (PA1068) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of PP2A-alpha using anti-PP2A-alpha antibody (PA1068).
PP2A-alpha was detected in a paraffin-embedded section of Rat Kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PP2A-alpha Antibody (PA1068) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PPP2CA (Source: Uniprot.org, NCBI)
Gene Name
PPP2CA
Full Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Weight
35594 MW
Superfamily
PPP phosphatase family
Alternative Names
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform;PP2A-alpha;3.1.3.16;Replication protein C;RP-C;PPP2CA; PPP2CA NEDLBA, PP2Ac, PP2CA, PP2Calpha, RP-C protein phosphatase 2 catalytic subunit alpha serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform|PP2A-alpha|protein phosphatase 2, catalytic subunit, alpha isozyme|replication protein C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP2CA, check out the PPP2CA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP2CA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PP2A-alpha/PPP2CA Antibody Picoband® (PA1068)
Hello CJ!
PA1068 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Tian Hp, Qiu Tz, Zhao J, Li Lx, Guo J. Brain Inj. 2009 Dec;23(13-14):1073-80. Doi: 10.3109/02699050903379388. Sphingomyelinase-Induced Ceramide Production Stimulate Calcium-Independent Jnk And Pp2A Activation Following Cerebral Ischemia.
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Customer Q&As
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6 Customer Q&As for Anti-PP2A-alpha/PPP2CA Antibody Picoband®
Question
Is there a BSA free version of anti-PP2A-alpha/PPP2CA antibody PA1068 available?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PP2A-alpha/PPP2CA antibody PA1068 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-10
Question
What's the concentration for PA1068 sample size? Thank you.
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
The concentration for PA1068 sample size is 200?g/ml.
Boster Scientific Support
Answered: 2019-08-23
Question
My lab would like to test anti-PP2A-alpha/PPP2CA antibody PA1068 on rat adrenal tissue for research purposes, then I may be interested in using anti-PP2A-alpha/PPP2CA antibody PA1068 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The products we sell, including anti-PP2A-alpha/PPP2CA antibody PA1068, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-17
Question
Is a blocking peptide available for product anti-PP2A-alpha/PPP2CA antibody (PA1068)?
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
We do provide the blocking peptide for product anti-PP2A-alpha/PPP2CA antibody (PA1068). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-01
Question
My question regarding product PA1068, anti-PP2A-alpha/PPP2CA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1068 anti-PP2A-alpha/PPP2CA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-26
Question
Will anti-PP2A-alpha/PPP2CA antibody PA1068 work for WB with adrenal tissue?
R. Jha
Verified customer
Asked: 2013-08-13
Answer
According to the expression profile of adrenal tissue, PPP2CA is highly expressed in adrenal tissue. So, it is likely that anti-PP2A-alpha/PPP2CA antibody PA1068 will work for WB with adrenal tissue.
Boster Scientific Support
Answered: 2013-08-13
