Anti-PIKE AGAP2 Antibody

Product Name

Anti-PIKE AGAP2 Antibody

View all Agap2 Antibodies

SKU/Catalog Number

A04771-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PIKE AGAP2 Antibody (Catalog # A04771-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.

Storage & Handling

PIKE antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PIKE AGAP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04771-1)

Host

Rabbit

Contents

PIKE Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PIKE antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human PIKE. The immunogen is located within the last 50 amino acids of PIKE.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.

Reactive Species

A04771-1 is reactive to AGAP2 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

124674 MW

Background of Agap2

Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04771-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PIKE antibody can be used for detection of PIKE by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For AGAP2 (Source: Uniprot.org, NCBI)

Alternative Names

PIKE, GGAP2, CENTG1, KIAA0167, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, Centaurin-gamma-1, AGAP-2 Agap2|Centg1, Pike|ArfGAP with GTPase domain, ankyrin repeat and PH domain 2|arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2|AGAP-2|centaurin, gamma 1|cnt-g1|nuclear GTPase PIKE|phosphatidylinositol 3-kinase enhancer

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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